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P1107

Sigma-Aldrich

JumpStart™ REDTaq® ReadyMix™ 反应混合物

for High-throughput PCR of complex templates

同義詞:

High throughput PCR, Hot start PCR master mix, Hot start master mix, Hot start taq master mix

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About This Item

MDL號碼:
MFCD00166026
分類程式碼代碼:
41106300
NACRES:
NA.55

品質等級

200

形狀

liquid

用途

sufficient for 100 reactions
 sufficient for 400 reactions

特點

Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart

濃度

0.75 units/reaction (50 μL reaction volume)

技術

PCR: suitable

顏色

red

輸入

purified DNA

應用

agriculture

運輸包裝

wet ice

儲存溫度

−20°C

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相關類別

一般說明

JumpStart REDTaq ReadyMix PCR反应混合物是制备好的溶液，结合了热启动PCR的性能优势和Sigma ReadyMix和REDTaq的双重便利性。反应混合物优化的2x反应浓缩物中包括Sigma的JumpStart Taq DNA聚合酶、99％纯度的脱氧核苷酸、缓冲液和惰性红色染料。将25 μL ReadyMix加到引物、模板和水中，使最终反应体积为50 μL。在室温下，反应混合物中的JumpStart Taq抗体可使Taq DNA聚合酶失活。在PCR的第一次变性步骤期间，复合物解离，聚合酶活性完全恢复。惰性红色染料不影响自动测序、限制性酶切、连接或其他下游操作。如果需要，只需通过标准纯化方法就可将PCR产物与染料轻松分离。热启动机制允许设置室温，使其成为高通量应用的理想选择 。
JumpStart REDTaq ReadyMix PCR Reaction Mix for High Throughput PCR is formulated with REDTaq Genomic DNA polymerase for amplification of more complex templates and genomic templates. This mix contains optimized enzyme and dye concentrations to provide increased length and yield on the more difficult templates.

應用

JumpStart™REDTaq®ReadyMix™ Reaction Mix has been used:
  • For multiplex PCR
  • For amplification with reduced non-specific amplification
  • For high throughput PCR amplification of complex templates
  • In the amplification of primers developed for the pathogenicity-associated genes of Clavibacter michiganensis
  • For PCR amplification of the human TMPRSS6 gene

特點和優勢

  • JumpStart™ REDTaq® DNA聚合酶是一种抗体失活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量和特异性
  • 无论模板浓度如何，JumpStart Taq DNA聚合酶均可提供优越的扩增效果
  • REDTaq JumpStart ReadyMix可减少移液步骤，降低污染风险。 热启动机制允许设置室温，使其成为高通量应用的理想选择 。
  • 惰性红色染料方便验证试剂是否适当混合
  • 不需要装载其他染料。可以在反应后将等分试样直接加载到琼脂糖凝胶上

包裝

默认的反应体积为50 μL

20RXN包装成1 X 500 μL
100RXN包装成1 X 2.5 mL
800RXN包装成1 X 20 mL

單位定義

在74℃下，一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。

其他說明

想查看JumpStart REDTaq和ReadyMix产品的更多详细信息，请访问 www.sigma-aldrich.com/hotstart。

法律資訊

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H317 - H412

危險分類

Aquatic Chronic 3 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCG4683
SLCH7361
SLCH3526
SLCB8123
SLBX7505

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Jarred Yasuhara-Bell et al.
International journal of systematic and evolutionary microbiology, 65(Pt 3), 811-826 (2014-12-08)
The genus Clavibacter contains one recognized species, Clavibacter michiganensis. Clavibacter michiganensis is subdivided into subspecies based on host specificity and bacteriological characteristics, with Clavibacter michiganensis subsp. michiganensis causing bacterial canker of tomato. Clavibacter michiganensis subsp. michiganensis is often spread through
Wenqin Wang et al.
BMC plant biology, 10, 205-205 (2010-09-18)
Members of the aquatic monocot family Lemnaceae (commonly called duckweeds) represent the smallest and fastest growing flowering plants. Their highly reduced morphology and infrequent flowering result in a dearth of characters for distinguishing between the nearly 38 species that exhibit
E Beutler et al.
Blood cells, molecules & diseases, 44(1), 16-21 (2009-10-13)
Male subjects with iron deficiency from the general population were examined for polymorphisms or sporadic mutations in TMPRSS6 to identify genetic risk factors for iron deficiency anemia. Three uncommon non-synonymous polymorphisms were identified, G228D, R446W, and V795I (allele frequencies 0.0074
John C Hafner et al.
Journal of biogeography, 38(6), 1077-1097 (2011-08-13)
AIM: The rodent genus Microdipodops (kangaroo mice) includes two sand-obligate endemics of the Great Basin Desert: M. megacephalus and M. pallidus. The dark kangaroo mouse, M. megacephalus, is distributed throughout the Great Basin and our principal aims were to formulate
'Touchdown' PCR to circumvent spurious priming during gene amplification.
R H Don et al.
Nucleic acids research, 19(14), 4008-4008 (1991-07-25)
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文章

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Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

條款

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

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