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R4775

Sigma-Aldrich

REDExtract-N-Amp™ PCR ReadyMix™

Ready-to-use 2X PCR Master Mix with Loading Dye

同義詞:

Direct plant PCR master mix, Direct tissue PCR master mix, PCR master mix with loading dye

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

品質等級

200

用途

sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
 sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
 sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

特點

dNTPs included
hotstart

顏色

red

應用

agriculture

運輸包裝

wet ice

儲存溫度

−20°C

相關類別

一般說明

REDExtract-N-Amp™ PCR反应混合物是包含缓冲液、盐、dNTP和REDTaq DNA聚合酶的PCR预混液。
该PCR ReadyMix要与Sigma的Extract-N-Amp植物PCR试剂盒以及Extract-N-Amp组织PCR试剂盒一起使用。所有Extract-N-Amp试剂盒均可为每次提取进行一次PCR反应。然而，如果需要更多的PCR反应，则可能需要补充额外的PCR ReadyMix。REDExtract-N-Amp PCR ReadyMix中含有一种惰性染料，可作为示踪染料以实现方便地将PCR产物加载到琼脂糖凝胶上进行分析。

應用

REDExtract-N-Amp™ PCR 预混液已被用于:
  • 固定的斑马鱼胚胎样品的基因分型
  • 使用REDExtract-N-Amp™植物PCR试剂盒对地衣家族中分离的DNA进行聚合酶链反应（PCR）扩增。
  • 使用REDExtract-N-Amp™种子PCR试剂盒扩增从单个酵母菌落和真菌孢子中分离的DNA。

REDExtract-N-Amp™ PCR 预混液还适用于菌落PCR。

特點和優勢

  • 即用型凝胶- 无需添加上样缓冲液或示踪染料。扩增后PCR产物直接上样到琼脂糖凝胶中 红色示踪剂的迁移速率与125bp片段大致相同（比溴酚蓝稍快）
  • 方便- JumpStart™ 产品中的热启动功能允许将反应设置在室温下，这在处理多个样品时非常有用。预混形式缩短了准备时间。
  • 可再现-降低多个移液步骤的污染风险，并在反应和时间方面具有一致的性能。
  • 高 特异性& 产量- JumpStart Taq DNA 聚合酶是一种抗体失活的热启动酶，设计用于最大限度降低非特异性扩增同时提高靶标的产量&特异性。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Prescott T Leach et al.
eNeuro, 3(1) (2016-03-30)
Fragile X syndrome is a neurodevelopmental disorder characterized by mild-to-severe cognitive deficits. The complete absence of Fmr1 and its protein product in the mouse model of fragile X (Fmr1 KO) provides construct validity. A major conundrum in the field is
Phylogenetic placement, species delimitation, and cyanobiont identity of endangered aquatic Peltigera species (lichen-forming Ascomycota, Lecanoromycetes).
Miadlikowska J
American Journal of Botany, 101(7), 1141-1156 (2014)
Amelia R Hofstetter et al.
PloS one, 11(2), e0149864-e0149864 (2016-02-26)
The role of the reactive oxygen species-producing NADPH oxidase family of enzymes in the pathology of influenza A virus infection remains enigmatic. Previous reports implicated NADPH oxidase 2 in influenza A virus-induced inflammation. In contrast, NADPH oxidase 1 (Nox1) was
Gil Benedek et al.
PLoS neglected tropical diseases, 15(10), e0009821-e0009821 (2021-10-19)
Nodding syndrome (NS) is a catastrophic and enigmatic childhood epilepsy, accompanied by multiple neurological impairments and neuroinflammation. Of all the infectious, environmental and psychological factors associated with NS, the major culprit is Onchocerca Volvulus (Ov)-a parasitic worm transmitted to human
Rasmus H Jensen et al.
Journal of clinical microbiology, 49(2), 549-556 (2010-12-15)
Candida palmioleophila has previously been misidentified as C. famata or C. guilliermondii. We have investigated traditional and modern identification methods for the identification of this and related species. Forty-one clinical isolates previously identified as C. famata or C. guilliermondii and
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文章

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

條款

REDExtract-N-Amp™ Plant PCR Kit Protocol

The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.

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