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Merck
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重要文件

C0549

Sigma-Aldrich

氯仿:异戊醇 24:1

suitable for nucleic acid purification

同義詞:

chloroform-isoamyl alcohol

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About This Item

分類程式碼代碼:
12190000
NACRES:
NA.52

形狀

liquid

包含

0.6-1.0% ethanol as stabilizer

適合性

suitable for nucleic acid purification

應用

agriculture

一般說明

氯仿:异戊醇 24:1混合液是一种用于 DNA 提取和沉淀的有机试剂。

應用

氯仿:异戊醇24:1混合液可用于：

  • 提取细菌基因组 DNA、水蚤(Daphnia) DNA 和鲑鱼精子。
  • 高质量 DNA 提取的单一 DNA 沉淀步骤。
  • 蛋白质提取和 RNA 酶处理。
  • 酚氧化，从水溶液中分离酚类物质。
  • 从棕色脂肪组织 (BAT)、腹股沟白色脂肪组织 (iWAT) 和附睾脂肪组织 (eWAT) 中分离 RNA。
  • 采用改良十六烷基三甲基溴化铵 (CTAB) 方法从凤仙花(Impatiens balsamina)中提取总 RNA 。

訊號詞

Danger

危險分類

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

標靶器官

Central nervous system, Liver,Kidney

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 3

閃點（°F）

185.0 °F

閃點（°C）

85 °C

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
MKCQ2693
MKCQ2692
MKCQ3155
MKCP5899
MKCP5902

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Optimisation of DNA extraction from the crustacean Daphnia.
Athanasio CG
PeerJ, 4, e2004-e2004 (2016)
Harnessing microbially generated power on the seafloor.
Tender LM
Nature Biotechnology, 20(8), 821-825 (2002)
Malte Thodberg et al.
Nucleic acids research, 47(4), 1671-1691 (2018-12-20)
Fission yeast, Schizosaccharomyces pombe, is an attractive model organism for transcriptional and chromatin biology research. Such research is contingent on accurate annotation of transcription start sites (TSSs). However, comprehensive genome-wide maps of TSSs and their usage across commonly applied laboratory
A fragrant neighborhood: volatile mediated bacterial interactions in soil.
Schulz-Bohm K
Frontiers in Microbiology, 6, 1212-1212 (2015)
Ning Jia et al.
Molecular cell, 75(5), 944-956 (2019-07-22)
Type III-A CRISPR-Cas surveillance complexes containing multi-subunit Csm effector, guide, and target RNAs exhibit multiple activities, including formation of cyclic-oligoadenylates (cAn) from ATP and subsequent cAn-mediated cleavage of single-strand RNA (ssRNA) by the trans-acting Csm6 RNase. Our structure-function studies have focused
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相關內容

DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

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