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P4557

Sigma-Aldrich

苯酚 溶液

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

同義詞:

Tris-equilabrated Phenol

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About This Item

經驗公式(希爾表示法):
C6H6O
CAS號碼:
分子量::
94.11
Beilstein:
6428463
EC號碼:
MDL號碼:
分類程式碼代碼:
12352104
PubChem物質ID:
NACRES:
NA.52

等級

for molecular biology

產品線

BioReagent

化驗

≥99.0% (GC)

形狀

liquid

pH值

7.7-8.1 (25 °C)

儲存溫度

2-8°C

SMILES 字串

Oc1ccccc1

InChI

1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H

InChI 密鑰

ISWSIDIOOBJBQZ-UHFFFAOYSA-N

基因資訊

human ... GABRA1(2554)

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一般說明

Tris 平衡苯酚溶液是一种透明液体,上面覆盖着一层 Tris 缓冲液。 苯酚溶液可用于多种分子生物学应用。苯酚等有机化合物具有芳香族碳环,其上直接连接有羟基。一羟基苯的通用名称是苯酚。

應用

苯酚溶液可用于:

  • 作为消化反应液组分制备不含RNA酶的质粒,用于兔网织红细胞裂解液的体外翻译
  • 从拟南芥莲座叶中提取 DNA
  • 从保存纸(preservation paper)上通过标准苯酚-氯仿法标准提取血液DNA
  • 作为提取缓冲液组分,提取果浆RNA
  • 从根中提取蛋白质
  • 分离粪便 DNA
  • 提取caged RNA

生化/生理作用

苯酚-氯仿法广泛用于核酸提取。尽管苯酚不能完全抑制 RNase 活性,但在与氯仿和乙醇联用时,其独特的特性有助于核酸分离。

成分

  • 1瓶Tris-平衡苯酚(货号P4557)
  • 平衡溶液(货号B5658)

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

標靶器官

Nervous system,Kidney,Liver,Skin

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


分析證明 (COA)

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