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等級
Molecular Biology
for molecular biology
化驗
≥99.0%
形狀
liquid
pH值
4.3-4.7
應用
life science and biopharma
儲存溫度
2-8°C
一般說明
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.
應用
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
- for the extraction and purification of RNA.
- to isolate RNA from Arabidopsis siliques.
- to extract proteins and DNA from polysomal and non-polysomal fractions.
- in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.
訊號詞
Danger
危險分類
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
標靶器官
Liver,Kidney, Nervous system,Kidney,Liver,Skin
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類（WGK）
WGK 3
閃點（°F）
175.0 °F - closed cup
閃點（°C）
79.44 °C - closed cup
個人防護裝備
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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條款
Ethidium Bromide Removal
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