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Sigma-Aldrich

Extract-N-Amp™植物PCR试剂盒

sufficient for 1000 extractions, sufficient for 1000 amplifications

同義詞:

Plant direct PCR kit

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 1000 amplifications
 sufficient for 1000 extractions

品質等級

200

特點

hotstart

技術

PCR: suitable

顏色

colorless

運輸包裝

wet ice

儲存溫度

−20°C

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相關類別

一般說明

Extract-N-Amp 植物PCR试剂盒含有从植物叶片中快速提取基因组DNA并通过PCR反应扩增感兴趣的目标基因必需的所有试剂。使用新型Extraction Solution，无需通过液氮对植物组织进行传统的冰冻，机械式破碎、有机萃取或沉淀DNA操作。该试剂盒同时包含一种专为从提取物直接扩增而配制的PCR ReadyMix™。

基因组DNA提取自0.5至0.7 cm的植物叶盘，该叶盘通过标准打孔器获得并在95 oC下Extraction Solution中孵育10分钟。向提取物中加入等体积的Dilution Solution，以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。
Extract-N-Amp™植物PCR试剂盒用于直接PCR（聚合酶链反应）。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案，无需常规的植物组织冷冻处理。

應用

Extract-N-Amp™植物PCR试剂盒适用于基因分型。

它已被用于从新鲜蘑菇中提取DNA。
Extract-N-Amp™组织PCR试剂盒用于从新鲜蘑菇中提取DNA。

特點和優勢

  • 可在15分钟内一步提取出用于PCR反应的植物基因组DNA
  • 专为直接从提取物进行扩增而配置的PCR ReadyMix™
  • 适用于基因组DNA的高度特异性PCR扩增的热启动抗体
  • 提取物可在4 °C 下至少稳定保存6个月
  • PCR用植物基因组DNA可以在15分钟内提取。
  • 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
  • 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
  • 热启动抗体确保基因组DNA的高度特异性PCR扩增
  • 适用于高通量植物基因分析
  • 无需长时间的酶消化，节省时间和精力
  • 提取的DNA在4 °C 下至少6个月保持稳定。

成分

Extract-N-Amp™植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物（2X）（缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体）。

其他說明

Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。
更多信息，请见www.sigma-aldrich.com/extract-n-amp。

法律資訊

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCL8334
SLCJ1522
SLCH7005
SLCH0353
SLCF9004

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存取文件庫

Antoine Lf Gady et al.
Plant methods, 5, 13-13 (2009-10-09)
The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as
Alison Heffer et al.
EvoDevo, 2(1), 7-7 (2011-03-03)
Tremendous progress has been made in the field of evo-devo through comparisons of related genes from diverse taxa. While the vast number of species in nature precludes a complete analysis of the molecular evolution of even one single gene family
M Riahi et al.
Genetics and molecular research : GMR, 9(3), 1334-1342 (2010-07-21)
Three DNA extraction protocols were compared for their ability to yield DNA from the leaves of herbarium specimens of nine species from nine genera of the Papilionoideae. We tested two protocols that use classic procedures for lysis and purification with
Dionne N Shepherd et al.
Journal of virological methods, 149(1), 97-102 (2008-02-19)
A high-throughput method of isolating and cloning geminivirus genomes from dried plant material, by combining an Extract-n-Amp-based DNA isolation technique with rolling circle amplification (RCA) of viral DNA, is presented. Using this method an attempt was made to isolate and
José Dijair Antonino de Souza Júnior et al.
PloS one, 8(12), e85364-e85364 (2014-01-07)
The root-knot nematode Meloidogyne incognita causes serious damage and yield losses in numerous important crops worldwide. Analysis of the M. incognita genome revealed a vast number of proteases belonging to five different catalytic classes. Several reports indicate that M. incognita
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文章

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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Extract-N-Amp™ Plant PCR Kits Protocol

Extract-N-Amp™ Plant PCR Kits Protocol

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