推薦產品
用途
sufficient for 70 purifications
品質等級
環保替代產品特色
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技術
DNA purification: suitable
環保替代類別
, Aligned
儲存溫度
15-25°C
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相關類別
一般說明
首先在液氮中研磨植物组织,在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作,去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤,进一步纯化基因组DNA。
纯化所得的DNA可直接用于PCR、限制性酶切、克隆、测序和Southern印迹等下游应用。
應用
- PCR
- 限制性内切酶酶切
- 克隆
- Southern印迹
- PCR
- 限制性内切酶酶解
- 克隆
- Southern印迹
GenElute™植物基因组DNA Miniprep试剂盒用于:
- 纯化从楝树组织中分离的基组DNA
- 从胚胎中提取基因组DNA
- 从植物样本中分离DNA
特點和優勢
- 起始材料:不超过100 mg的植物组织
- 预期得率:至多20 μg
- 洗脱体积:100 - 200 μl
- 所需时间:<40分钟
- 是否需要RNase处理:否
原則
其他說明
法律資訊
推薦
訊號詞
Danger
危險分類
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C
安全危害
儲存類別代碼
8A - Combustible corrosive hazardous materials
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
條款
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
相關內容
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
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