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一般說明
GenElute 细菌基因组DNA试剂盒可以简单、方便地从革兰氏阴性菌中分离高纯度基因组DNA。对于大多数革兰氏阳性菌,本试剂盒必须结合选购的溶菌酶 (L4919)来有效裂解较厚的肽聚糖细胞壁。为方便您制备溶菌酶储液,我们提供了一种稀释的革兰氏阳性溶解溶液。
该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起,同时无需采用昂贵的树脂、乙醇沉淀,以及诸如苯酚和氯仿等有害化学成分。
该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起,同时无需采用昂贵的树脂、乙醇沉淀,以及诸如苯酚和氯仿等有害化学成分。
應用
纯化所得的细菌基因组DNA可用于以下游应用:
- 限制性内切酶酶切
- PCR
- Southern印迹
- 克隆
特點和優勢
- 起始材料:不超过1.5 mL培养物
- 预期得率:至多20 μg
- 洗脱体积:400 μl
- 所需时间:70 - 120分钟
- A260/A280比率:1.6 - 1.9
- 无需苯酚、氯仿或乙醇沉淀步骤
原則
细菌首先在含有离液盐的溶液之中裂解,以确保大分子彻底变性。之后加入乙醇,从而使DNA在溶解物通过微量离心管在硅胶膜上离心时,结合到膜上。在洗涤去除污染物后,将DNA放在200 μL的Tris-EDTA溶液中洗脱。
预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间,最大长度可达50 kb。
预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间,最大长度可达50 kb。
其他說明
更多信息,请见www.sigma-aldrich.com/genomicdna。
法律資訊
GenElute is a trademark of Sigma-Aldrich Co. LLC
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產品號碼
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危險分類
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Central nervous system, Respiratory system
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3 - Flammable liquids
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GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
GenElute Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
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