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NA1020

Sigma-Aldrich

GenElute™ PCR 纯化试剂盒

sufficient for 70 purifications

同義詞:

Gen Elute, PCR纯化

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.52

用途

sufficient for 70 purifications

品質等級

200

技術

DNA purification: suitable

儲存溫度

15-25°C

相關類別

一般說明

GenElute PCR回收试剂盒可用于从各种其他反应组分（如多余的引物、核苷酸、DNA聚合酶、油和盐）之中快速纯化出单链或双链PCR扩增产物（长度100 bp至10 kb）。该试剂盒将硅胶结合技术的优势与传统离心柱形式结合在一起，同时无需采用昂贵的树脂或苯酚和氯仿等有毒有机化学成分。
GenElute PCR回收试剂盒可用于从各种其他反应组分（如多余的引物、核苷酸、DNA聚合酶、油和盐）之中快速纯化出单链或双链PCR扩增产物（长度100 bp至10 kb）。该试剂盒将硅胶结合技术的优势与传统离心柱形式结合在一起，同时无需采用昂贵的树脂或苯酚和氯仿等有毒有机化学成分。

DNA结合到离心柱内的硅胶膜上。结合所得的DNA经过洗涤后，再在所选的缓冲液中洗脱所得的洁净、浓缩的DNA。每个离心柱至多可纯化100 μL或10 μg经PCR扩增的DNA，至多可纯化出 95%的100 bp至10 kb大小的PCR产物。该试剂盒可去除99%以上的引物和大多数的引物二聚体(<40 bp)。

纯化所得的DNA可用于酶促反应、传统或自动化测序、克隆和微阵列分析。

應用

GenElute™ PCR产物纯化试剂盒已被用于从反应的其他组分中快速纯化单链或双链PCR扩增产物（100bp至10kb）。
纯化所得的DNA可用于酶促反应、传统或自动化测序、克隆和微阵列分析。

特點和優勢

  • 可回收95%长度介于100 bp至10 kb之间的PCR产物
  • 可在8分钟内纯化出多达100 μL或10 μg 的PCR扩增产物DNA。
  • 可去除99%以上的引物和其他成分
  • 可在8分钟内纯化出多达100 μl或10 μg 的PCR扩增产物DNA。
  • 可回收95%长度介于100 bp至10 kb之间的PCR产物
  • 可去除99%以上的引物和其他成分
  • 不需要去除有机萃取引入的矿物油
  • 相对于其他市场领先竞争对手，可多获得40%的纯化制备物

其他說明

GenElute PCR回收试剂盒可用于从各种其他反应组分（如多余的引物、核苷酸、DNA聚合酶、油和盐）之中快速纯化出单链或双链PCR扩增产物（长度100 bp至10 kb）。该试剂盒将硅胶结合技术的优势与传统离心柱形式结合在一起，同时无需采用昂贵的树脂或苯酚和氯仿等有毒有机化学成分。

原則

GenElute PCR回收试剂盒将硅胶结合和微量离心形式的优势结合在一起。DNA结合到离心柱内的硅胶膜上。结合所得的DNA经过洗涤后，再在所选的缓冲液中洗脱所得的洁净、浓缩的DNA。每个离心柱至多可纯化100 μL或10 μg经PCR扩增的DNA，至多可纯化出 95%的100 bp至10 kb大小的PCR产物。该试剂盒可去除99%以上的引物和大多数的引物二聚体(可去除<40 bp的片段)。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

象形圖

CorrosionExclamation mark
GHS05,GHS07

訊號詞

Warning

危險分類

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
SLCL6932
SLCG3825
SLCG3826
SLCD4369
SLCD3370

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Andreas Veith et al.
Frontiers in microbiology, 2, 37-37 (2011-07-13)
The sulfur oxygenase reductase (SOR) is the initial enzyme of the sulfur oxidation pathway in the thermoacidophilic Archaeon Acidianus ambivalens. The SOR catalyzes an oxygen-dependent sulfur disproportionation to H(2)S, sulfite and thiosulfate. The spherical, hollow, cytoplasmic enzyme is composed of
Isolation of disseminated neuroblastoma cells from bone marrow aspirates for pretreatment risk assessment by array comparative genomic hybridization.
Vandewoestyne M
International Journal of Cancer. Journal International Du Cancer, 130(5), 1098-1108 (2012)
Kathleen Gärtner et al.
Retrovirology, 6, 32-32 (2009-04-08)
Foamy viruses (FVs) are the most genetically stable viruses of the retrovirus family. This is in contrast to the in vitro error rate found for recombinant FV reverse transcriptase (RT). To investigate the accuracy of FV genome copying in vivo
Elisa M Salas et al.
Genes & cancer, 2(5), 593-596 (2011-09-09)
A search for genes potentially regulated by STAT5 identified leukemia inhibitory factor (LIF) as a good candidate. Using various experimental approaches, we have validated LIF as a direct transcriptional target of STAT5 in myeloid cell lines: STAT5 binds to LIF
Charlotte G Cole et al.
Genome biology, 9(5), R78-R78 (2008-05-15)
Although the human genome sequence was declared complete in 2004, the sequence was interrupted by 341 gaps of which 308 lay in an estimated approximately 28 Mb of euchromatin. While these gaps constitute only approximately 1% of the sequence, knowledge
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