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一般說明
GenElute哺乳动物基因组DNA纯化试剂盒可以简单、方便地从多种哺乳动物源样本中分离高纯度的大分子量DNA。此类试剂盒使用了为基因组DNA纯化精选的硅胶膜，采用了方便的离心柱形式。
可从培养细胞、组织（包括啮齿动物尾巴）以及新鲜的全血或白血细胞之中分离高纯度的基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。
可从培养细胞、组织（包括啮齿动物尾巴）以及新鲜的全血或白血细胞之中分离高纯度的基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。
應用
纯化所得的基因组DNA可直接供以下下游应用使用：
- 限制性内切酶酶切
- PCR
- Southern印迹
- 测序反应
- 克隆
- 连接
GenElute™ Mammalian Genomic DNA Miniprep Kits has been used:
- to extract DNA from fin samples
- to extract the BSTUI restriction enzyme from the DNA
- to isolate DNA from the blood samples extracted from caudal fin
- to extract tissue and hair follicle DNA
特點和優勢
- 预期得率：25 μg / 2 x 106个培养细胞；30 μg / 25 mg组织
- 洗脱体积：200 - 400 μl
- 所需时间：细胞裂解后20分钟
- A260/A280比率：1.6 - 1.9
- 是否需要机械式均质化：否
原則
起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，裂解物离心通过微量离心管时，DNA会结合到硅胶模上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。
其他說明
更多信息，请见www.sigma-aldrich.com/genomicdna。
法律資訊
GenElute is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
8A - Combustible corrosive hazardous materials
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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條款
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
The GenElute Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
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