T0956

Sigma Universal Transfection Reagent

Polymer reagent for transfecting common mammalian cell lines

• 分類程式碼代碼: 12352202

屬性

• 等級: for molecular biology
• 品質等級: 200
• 無菌: aseptically filled
• 形狀: liquid (buffered aqueous solution)
• 用途: sufficient for 110 transfections (3.5 cm dish); sufficient for 40 transfections (10 cm dish); sufficient for 65 transfections (6 cm dish)
• 濃度: 2 mg/mL
• 儲存溫度: −20°C

描述

一般說明

Sigma Universal Transfection Reagent is a unique formulation of a biodegradable, proprietary cationic polymer conjugated to a cationic lipid. Universal Transfection Reagent is used for transfection of nucleic acids into common eukaryotic cell lines. Fast and easy protocol is compatible with serum, serum-free medium and antibiotics. For most cells, medium change after transfection is not necessary.

應用

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 1-3 μl Universal Transfection Reagent per μg DNA. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Sigma Universal Transfection Reagent:

Excellent for:
BHK-21
CHO
CHOK1
HEK293
HeLa
NIH3T3

Good for:
3LL
6CSFME
ATT20B16-F0
B16-F10
BAEC
Ca Ski
COS-7
CV-1
D 407
HUVEC
L929
MCF-7
MDCK
Neuro2A
SAOS-2
SHEP
SiHa
SKOV3

特點和優勢

• 简便、快速的实验方案
• 适于稳定和瞬时转染
• 对于包括原代细胞在内的广泛细胞类型，均具有高转染效率
• 兼容含血清和不含血清的转染实验

成分

Note the formulation of Sigma Universal Transfection Reagent is confidential.

原則

Sigma Universal Transfection Reagent is a cationic polymer conjugated to a cationic lipid. When mixed with plasmid DNA, the reagent forms stable complexes that can be directly added to the cell culture. The cells take up the complexes by endocytosis, the polymer is cleaved from the complex, and the plasmid DNA is quickly released into the cytoplasm.

其他說明

Complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum lowers the toxicity of transfection.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable