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重要文件

NA0200

Sigma-Aldrich

GenElute HP质粒中量制备试剂盒

greener alternative

sufficient for 25 purifications

同義詞:

GenElute HP质粒中量制备试剂盒, Gen Elute

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.52

用途

sufficient for 25 purifications

品質等級

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

DNA purification: suitable

環保替代類別

儲存溫度

15-25°C

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一般說明

GenElute HP质粒中量制备试剂盒可以超快速、高效的从重组大肠杆菌培养物大批量制备质粒。该试剂盒结合了硅胶膜技术和离心或真空形式的便利性。该试剂盒还附带专用的滤器卡夹,从而替代了碱裂解之后的离心分离步骤。裂解之后,DNA结合到硅胶膜上,并通过一次简单的洗涤步骤去除了污染物。随后在水或TE缓冲液中,通过离心处理洗脱DNA。

纯化所得的质粒DNA适于包括限制性酶切和测序在内的一系列分子生物学应用。
我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。相比标准DNA提取方法使用苯酚和氯仿,该产品是一种更安全的化学产品。

應用

GenElute HP 质粒Midiprep试剂盒已用于分离和纯化质粒。

特點和優勢

  • 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
  • 可纯化出多达350 μg高拷贝数质粒DNA
  • 真空或离心形式可灵活选择
  • 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
  • 所含的塑料成分更少,减少了废物量

其他說明

更多信息,请见www.sigma-aldrich.com/genelutehp

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

3 - Flammable liquids


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Vanessa E Gray et al.
G3 (Bethesda, Md.), 9(11), 3683-3689 (2019-09-29)
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The distal upstream promoter in Ly49 genes, Pro1, is active in mature NK cells and T cells, does not require TATA boxes, and displays enhancer activity.
Gays F
Journal of Immunology, 194(12), 6068-6081 (2015)
Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Steinhart Z
Nature Medicine, 23(1), 60-68 (2017)
Stephanie P Cartwright et al.
BMC research notes, 10(1), 340-340 (2017-07-29)
Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV
Ping Wang et al.
Autophagy, 16(1), 123-139 (2019-03-27)
Autophagy is a conserved catabolic process in eukaryotes that contributes to cell survival in response to multiple stresses and is important for organism fitness. In Arabidopsis thaliana, the core machinery of autophagy is well defined, but its transcriptional regulation is

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相關內容

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

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