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L3287

Sigma-Aldrich

Escort ™ IV 转染试剂

Lipid reagent for transient and stable transfection of mammalian and insect cells.

同義詞:

Gene delivery

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About This Item

分類程式碼代碼:
41106502
NACRES:
NA.25

等級

for molecular biology

品質等級

100

形狀

liquid (aqueous solution)

用途

1 mL sufficient for 160-500 transfections

濃度

1 mg/mL

技術

transfection: suitable

儲存溫度

2-8°C

相關類別

一般說明

Escort™ IV是由专利的聚阳离子脂质体和中性非转染脂质体构成的独特配方。这种脂质体构成的化合物可用于向不同类型的真核细胞转染核酸。

應用

适用于将核酸瞬时和稳定转染到培养的真核细胞中。 每 6 cm 细胞培养板使用约 4-16μL Escort™ IV 和 2μg DNA。方案优化提供了非常高效的转染。关于使用 Escort ™ IV 成功转染的细胞列表，请参见 转染试剂选择指南 。

特點和優勢

  • 适于稳定和瞬时转染
  • 针对广泛的细胞类型进行了优化
  • 低细胞毒性
  • 兼容含血清和不含血清的转染实验
  • 非常适于 Sf9, Sf21 和S2昆虫细胞

成分

Escort™ IV 配方:
1 mg/mL溶于水的总脂质体

请注意，Escort™ IV 中使用的脂质体具体类别属于机密信息。

注意

注意：不要冻存。

原則

在不存在血清条件下，将Escort™ IV与DNA混合，即可形成稳定的复合物。该复合物性质稳定，可直接加入到细胞培养基中，并在培养基中与细胞膜融合，将DNA释放到细胞质中。注意：复合物的形成受到血清抑制，但一旦形成稳定的复合物，即便存在血清也无妨。

法律資訊

Escort is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

分析證明 (COA)

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Michael Povelones et al.
PLoS pathogens, 7(4), e1002023-e1002023 (2011-05-03)
Malaria threatens half the world's population and exacts a devastating human toll. The principal malaria vector in Africa, the mosquito Anopheles gambiae, encodes 24 members of a recently identified family of leucine-rich repeat proteins named LRIMs. Two members of this
H A Clark et al.
Analytical chemistry, 71(21), 4831-4836 (1999-11-24)
Spherical optical nanosensors, or PEBBLEs (probes encapsulated by biologically localized embedding), have been produced in sizes including 20 and 200 nm in diameter. These sensors are fabricated in a microemulsion and consist of fluorescent indicators entrapped in a polyacrylamide matrix.
Zev Bryant et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(3), 772-777 (2006-12-22)
Myosin VI supports movement toward the (-) end of actin filaments, despite sharing extensive sequence and structural homology with (+)-end-directed myosins. A class-specific stretch of amino acids inserted between the converter domain and the lever arm was proposed to provide
Manveen K Gupta et al.
Antioxidants & redox signaling, 8(5-6), 1081-1093 (2006-06-15)
Cardiac myocytes, upon exposure to increasing doses of norepinephrine (NE), transit from hypertrophic to apoptotic phenotype. Since reactive oxygen species (ROS) generation is attributed to both phenomena, the authors tested whether an elevation in intracellular ROS level causes such transition.
Melissa D Jordan et al.
Chemical senses, 34(5), 383-394 (2009-03-19)
Moths recognize a wide range of volatile compounds, which they use to locate mates, food sources, and oviposition sites. These compounds are recognized by odorant receptors (OR) located within the dendritic membrane of sensory neurons that extend into the lymph
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Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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