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NA0310

Sigma-Aldrich

GenElute™ HP质粒大量制备试剂盒

greener alternative

sufficient for 25 purifications

同義詞:

GenElute™ HP质粒大量制备试剂盒

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.52

用途

sufficient for 25 purifications

品質等級

200

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

DNA purification: suitable

環保替代類別

Aligned,

儲存溫度

15-25°C

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相關類別

一般說明

GenElute HP质粒大量制备试剂盒可以简单、快速、低成本的从重组大肠杆菌培养物中分离质粒DNA。该试剂盒采用了一种可快速清除裂解液的过滤针筒和一种适于真空或离心形式的硅胶结合柱。从150 mL Luria Broth (LB)培养基过夜培养物中，可分离出多达1.2 mg 的质粒DNA。请注意，实际得率取决于所用的菌株、质粒和培养基。

通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。通过两遍洗涤步骤，去除污染物。最后，在洗脱溶液(Tris-HCl)或水中洗脱结合所得的DNA。

回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接供限制性酶切、连接、测序、PCR、转化和转染等下游应用使用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，该产品是一种更安全的化学产品。

應用

GenElute® HP Plasmid Maxiprep试剂盒已用于分离质粒。

特點和優勢

  • 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
  • 可纯化出多达1.2 mg的高拷贝数质粒DNA
  • 真空或离心形式可灵活选择
  • 相对于其他快速试剂盒，所含的塑料成分更少，减少了废物量
  • 无需进行苯酚/氯仿萃取或乙醇沉淀步骤

其他說明

更多信息，请见www.sigma-aldrich.com/genelutehp。

法律資訊

Gabitril is a registered trademark of Abbott Laboratories Corp.
GenElute is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

3 - Flammable liquids

閃點（°F）

77.0 °F - closed cup

閃點（°C）

25 °C - closed cup

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Jaeho Yoon et al.
Biomedicines, 9(9) (2021-09-29)
Renal hypouricemia is a rare genetic disorder. Hypouricemia can present as renal stones or exercise-induced acute renal failure, but most cases are asymptomatic. Our previous study showed that two recessive variants of SLC22A12 (p.Trp258*, pArg90His) were identified in 90% of
Musaddeque Ahmed et al.
Nature communications, 12(1), 1781-1781 (2021-03-21)
Prostate cancer (PCa) risk-associated SNPs are enriched in noncoding cis-regulatory elements (rCREs), yet their modi operandi and clinical impact remain elusive. Here, we perform CRISPRi screens of 260 rCREs in PCa cell lines. We find that rCREs harboring high risk
Discovery of protein interactions using parallel analysis of translated ORFs (PLATO).
Larman HB
Nature Protocols, 9(1), 90-103 (2014)
Fraser Soares et al.
Blood, 137(16), 2171-2181 (2020-12-04)
Acute myeloid leukemia (AML) remains a devastating disease in need of new therapies to improve patient survival. Targeted adoptive T-cell therapies have achieved impressive clinical outcomes in some B-cell leukemias and lymphomas but not in AML. Double-negative T cells (DNTs)
Ilaria Mogno et al.
Genome research, 23(11), 1908-1915 (2013-08-08)
Gene promoters typically contain multiple transcription factor binding sites (TFBSs), which may vary in affinity for their cognate transcription factors (TFs). One major challenge in studying cis-regulation is to understand how TFBS variants affect gene expression. We studied the in
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GenElute™ HP Plasmid Maxiprep procedure

The GenElute™ HP Plasmid Maxiprep kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cutures. The recovered plasmid DNA is ready for immediate use in downstream applications, such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.

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