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G1N10

Sigma-Aldrich

GenElute™ 哺乳动物基因组DNA小量制备试剂盒

sufficient for 10 purifications

同義詞:

哺乳动物基因组DNA小量制备, Gen Elute

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.55

用途

sufficient for 10 purifications

儲存溫度

15-25°C

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相關類別

一般說明

GenElute哺乳动物基因组DNA纯化试剂盒可以简单、方便地从多种哺乳动物源样本中分离高纯度的大分子量DNA。此类试剂盒使用了为基因组DNA纯化精选的硅胶膜，采用了方便的离心柱形式。

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，当裂解物离心通过微量离心管时DNA会结合在硅胶膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

纯化所得的基因组DNA可直接用于限制性酶切、PCR、Southern印迹、测序反应等下游应用。

應用

纯化所得的基因组DNA可直接供以下下游应用使用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆
  • 连接

特點和優勢

  • 预期得率：25 μg / 2 x 106个培养细胞；30 μg / 25 mg组织
  • 洗脱体积：200 - 400 μl
  • 所需时间：细胞裂解后20分钟
  • A260/A280比率：1.6 - 1.9
  • 是否需要机械式均质化：否

原則

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，裂解物离心通过微量离心管时，DNA会结合到硅胶模上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他說明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • C2112Column Preparation SolutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS
  • R6148RNase A solutionSDS

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訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

483.8 °F

閃點（°C）

251 °C

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCL7191
SLCK8229
SLCF6078
SLCC7185
SLCB3846

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存取文件庫

Matthew J Coussens et al.
Journal of visualized experiments : JoVE, (62)(62), e3303-e3303 (2012-04-18)
The Target ID Library is designed to assist in discovery and identification of microRNA (miRNA) targets. The Target ID Library is a plasmid-based, genome-wide cDNA library cloned into the 3'UTR downstream from the dual-selection fusion protein, thymidine kinase-zeocin (TKzeo). The
Yuma Yamada et al.
Biomaterials, 33(15), 3952-3958 (2012-03-06)
A quantitative comparison between nuclear DNA release from carriers and their transfection activity would be highly useful for improving the effectiveness of non-viral gene vectors. We previously reported that, for condensed DNA particles, a close relationship exists between the efficiency
Coralie Bertheau et al.
Molecular ecology, 22(12), 3318-3332 (2013-05-29)
Ips typographus and Pityogenes chalcographus are two sympatric Palearctic bark beetle species with wide distribution ranges. As both species are comparable in biology, life history, and habitat, including sharing the same host, Picea abies, they provide excellent models for applying
Giulia Crispino et al.
Scientific reports, 7(1), 6567-6567 (2017-08-06)
We have previously shown that in vitro transduction with bovine adeno-associated viral (BAAV) vectors restores connexin expression and rescues gap junction coupling in cochlear organotypic cultures from connexin-deficient mice that are models DFNB1 nonsyndromic hearing loss and deafness. The aims
Michal Kirshner et al.
Journal of molecular neuroscience : MN, 46(3), 554-568 (2011-09-17)
Pronounced neuropathology is a feature of ataxia-telangiectasia (A-T) and Nijmegen breakage syndrome (NBS), which are both genomic instability syndromes. The Nbs1 protein, which is defective in NBS, is a component of the Mre11/RAD50/NBS1 (MRN) complex. This complex plays a major
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