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WGA1

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GenomePlex® Whole Genome Amplification (WGA) Kit

Kit for whole genome amplification from a variety of DNA sources including FFPE tissue

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About This Item

分類程式碼代碼:
41121800
NACRES:
NA.55

品質等級

200

技術

whole genome amplification: suitable

運輸包裝

wet ice

儲存溫度

−20°C

相關類別

一般說明

GenomePlex® 全基因组扩增 WGA 试剂盒适用于基因组DNA、全血、白细胞层（buffy coat）、口腔拭子和培养的细胞。 扩增只需要少量起始原料（1 μL 血、单面颊拭子或10 ng gDNA），PCR后每次反应可得到5 - 10 μg的产量 。 扩增的DNA 可进行纯化和储存或用于下游分析，包括ABI′s TaqMan®测定、微卫星分析或测序。 GenomePlex 完整表达整个基因组，等位基因偏差最小。

WGA试剂盒采用基于基因组 DNA 随机片段化的专有技术，并将产生的小片段转换为两侧有通用引物结合位点的 PCR 可扩增文库分子。 WGA是利用通用寡核苷酸引物对文库分子进行PCR扩增获得的。

GenomePlex 方法让研究人员可以生成具有代表性的、约 500 倍扩增的基因组 DNA 。 扩增产量取决于纯度和起始原料的量。该试剂盒含有进行文库制备和片段化需要的所有试剂。

應用

GenomePlex ® 全基因组扩增 (WGA) 试剂盒已用于扩增输入和染色质免疫沉淀 (ChIP) DNA。它也用于来源于细菌培养物的细菌人工染色体（BAC）克隆体的片段化，用于制备无重复（repeat free，RF）探针。

特點和優勢

  • 以最少的模板获得更高的产量: 在三小时以内将纳克量（10ng）的基因组DNA扩增为微克产量（5-10 μg）
  • 纳克量样品可在–20 °C下保存备用
  • 从各种DNA来源中选择：全血、口腔拭子、 血卡、植物、土壤、福尔马林固定石蜡包埋组织（FFPE）
  • 兼容各种下游应用，如TaqMan®和BeadArray 测定
  • 通用引物
  • 表达全基因组，没有可检测的偏差
  • 增加的扩增准确性不会在阴性对照反应中产生扩增子

其他說明

该试剂盒随附的通用引物的序列组成具有专利权。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

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產品號碼
描述
SDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Adam Hittelman et al.
Diagnostic molecular pathology : the American journal of surgical pathology, part B, 16(4), 198-206 (2007-11-29)
Genome-based technologies such as genomic arrays and next generation sequencing are poised to make significant contributions to clinical oncology. However, translation of these technologies to the clinic will require that they produce high-quality reproducible data from small archived tumor specimens
Amy Breman et al.
Prenatal diagnosis, 32(4), 351-361 (2012-04-03)
To evaluate the results of prenatal chromosomal microarray analysis (CMA) on >1000 fetal samples referred for testing at our institution and to compare these data to published reports. High resolution CMA was offered to women undergoing amniocentesis or chorionic villus
Kaye N Ballantyne et al.
Forensic science international, 166(1), 35-41 (2006-05-12)
The analysis of LCN or highly degraded DNA samples presents a challenge for forensic science. Improving the quantity and/or quality of samples would greatly increase the profiling success rate from LCN and degraded samples. Whole genome amplification (WGA) is one
Nicola A Johnson et al.
Laboratory investigation; a journal of technical methods and pathology, 86(9), 968-978 (2006-06-06)
Array-based comparative genomic hybridisation (aCGH) has diverse applications in cancer gene discovery and translational research. Currently, aCGH is performed primarily using high molecular weight DNA samples and its application to formalin-fixed and paraffin-embedded (FFPE) tissues remains to be established. To
Binding patterns of BCL11A in the globin and GATA1 loci and characterization of the BCL11A fetal hemoglobin locus
Jawaid K, et al.
Blood Cells, Molecules and Diseases, 45(2), 140-146 (2010)
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條款

Whole Gene Amplification Formalinfixed Paraffin embedded Tissues

Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases.

Protocol for DNA extraction & WGA Amplification from Soil Samples

Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the GenomePlex® Whole Genome Amplification Protocol is followed

Saliva DNA Extraction & WGA Amplification Protocol

This protocol provides a simple and convenient method to isolate, amplify and purify genomic DNA from saliva

Extraction & Amplification of Whole Blood Using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

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相關內容

GenomePlex Whole Genome Amplification Kit Protocol (WGA1)

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.

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