Whole Genome Amplification

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Whole Genome Amplification (WGA) Kits

Genomic testing and characterization have become an important tool for understanding biological systems. Often, such analysis is hampered by the number of samples to be examined and the availability of sufficient quantities of genomic DNA. This is particularly a problem for rare and archived sources of DNA. The GenomePlex^® Whole Genome Amplification (WGA) kits have been developed for use as a high-throughput system for the rapid and highly representative, amplification of genomic DNA from trace amounts of starting material.

The GenomePlex^® WGA kits utilize a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex^® Library molecules flanked by universal priming sites. The OmniPlex^® library is then amplified using universal oligonucleotide primers and a limited number of cycles. The GenomePlex^® WGA kits require nanogram quantities of genomic DNA with yields of 5-10 µg following PCR. This system is suitable with a variety of purified DNA samples including whole blood, blood cards, buccal swabs, plant sources, and formalin-fixed, paraffin-embedded tissue samples.

Single Cell Whole Genome Amplification Strategies

The GenomePlex^® Single Cell Whole Genome Amplification Kit (WGA4) is designed to amplify the genome of a single cell resulting in a million-fold amplification yielding microgram quantities of genomic DNA. Traditional single cell whole genome amplification methods yield insufficient quantities with significant biased representation. However, the GenomePlex^® technology provides amplification efficiency with WGA DNA Polymerase. The Single Cell WGA kit includes all the reagents necessary for cell lysis and subsequent whole genome amplification.

The Single Cell WGA kit has been validated with numerous downstream applications and is suitable for amplification from a variety of sources including plant, polycarbonate fixed cells, lymphocytes, renal cancer cells, epithelial cells, leukemia cells, and fibroblast amniotic cells. The amplified genome is compatible with many downstream applications including comparative genomic hybridization (CGH), microsatellite/STR analysis, SNP analysis, QPCR, and other genotyping analysis.

Whole Transcriptome Amplification

Whole transcriptome amplification (WTA) kits have been developed to generate sufficient transcript targets from minute amounts of RNA. Successful WTA requires accurate replication of representative transcripts present in a sample – without dropout or bias of specific mRNAs. Our WTA kits provide an accurate, fast and simple method of amplifying total RNA from a variety of sources including blood, fixed and frozen tissue, cell culture, FACS sorted cells, plants and microorganisms. Additionally, the WTA (WTA1, WTA2, SeqR) kits provide rapid amplification of total RNA in less than 4 hours without 3’-bias. Amplified RNA (cDNA) is suitable for qPCR, micro array analysis, and traditional cloning. For additional information, please visit our WTA1, WTA2, or SeqR product pages.

SeqPlex-I DNA and RNA Amplification Kits for Illumina^® Next Generation Sequencing

The SeqPlex-I DNA and RNA Amplification Kits for whole genome amplification (WGA) and whole transcriptome amplification (WTA) are designed to facilitate Illumina^® next-generation sequencing (NGS) from extremely small quantities or degraded/highly fragmented DNA and RNA. Both the WGA and WTA kits utilize a proprietary technology based on random priming of genomic DNA or RNA and conversion of the resulting small fragments to PCR amplifiable library molecules flanked by universal priming sites. The SeqPlex-I WGA and WTA kits allow easy amplification of limited starting samples for convenient direct input onto Illumina^® NGS flow cells, enabling you to accelerate your next big discovery.