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PKH67GL

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Kit，用于常规细胞膜标记

Distributed for Phanos Technologies

同義詞:

Green PKH membrane labeling kit

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.32

品質等級

200

包裝

pkg of 1 kit

儲存條件

protect from light

螢光

λex 490 nm; λem 502 nm (PKH67 dye)

檢測方法

fluorometric

運輸包裝

ambient

儲存溫度

room temp

相關類別

應用

本试剂盒适用于常规细胞膜标记。PKH67具有长于PKH1和PKH2的脂肪炭尾，后两者是另外两种之前常用于体外和体内细胞示踪的绿色染料。由于炭尾长度更长，内部研究已经证明PKH67比PKH2由更少的细胞间转移。
在采用PKH1和PKH2进行的体内研究中，荧光强度都会缓慢损失。由于这是绿色细胞linker染料而非红色细胞linker染料出现的行为特征，因而PKH67会出现类似的性质。不分裂细胞的体外细胞膜留存和体内荧光半衰期的关联性揭示，PKH67的体内荧光半衰期为10-12天。其他具有类似半衰期的绿色细胞linker染料已经被用于监测1-2月内的体内 淋巴细胞和巨噬细胞运输，结果表明PKH67还可用于中等时长的体内跟踪研究。
用于常规细胞膜标记的PKH26红色荧光细胞连接子试剂盒已用于：
  • 标记并进一步研究在三阴性乳腺癌状况下从细胞中排出的外泌体。
  • 标记凋亡性细胞，用于研究ανβ5受体在凋亡性细胞的结合和内化中的作用。
  • 在荧光成像中。

聯結

如需关于PKH及CellVue®的荧光细胞连接子染料的更多技术详情，包括大量的参考书目，请访问这里。

法律資訊

CellVue is a registered trademark of Phanos Technologies

僅套裝組件

產品號碼
描述
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

象形圖

FlameExclamation mark
GHS02,GHS07

訊號詞

Danger

危險聲明

H225,H319

危險分類

Eye Irrit. 2 - Flam. Liq. 2

儲存類別代碼

3 - Flammable liquids

閃點（°F）

57.2 °F - closed cup

閃點（°C）

14 °C - closed cup

分析證明 (COA)

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alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)
Increased expression of inducible HSP70 in apoptotic cells is correlated with their efficacy for antitumor vaccine therapy
Masse D, et al.
International Journal of Cancer. Journal International Du Cancer, 111(4), 575-583 (2004)
Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Z M Wu et al.
Placenta, 33(3), 188-194 (2012-01-04)
As human blastocyst-derived extravillous trophoblasts (EVTs) invade the early decidua, they are positioned to interact with immune cells and resident decidual cells, and remodel spiral arteries into high capacity vessels that increase blood flow to the developing fetal-placental unit. Shallow
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