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G2N10

Sigma-Aldrich

GenElute™ 植物基因组 DNA 微量制备试剂盒

greener alternative

sufficient for 10 purifications

同義詞:

植物基因组DNA少量制备, Gen Elute

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.55

用途

sufficient for 10 purifications

環保替代產品特色

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

環保替代類別

Aligned,

儲存溫度

15-25°C

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相關類別

一般說明

GenElute植物基因组DNA小量制备试剂盒可以简单、方便的从各种植物中分离高纯度的DNA。GenElute试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、RNase处理，以及诸如苯酚和氯仿等有害化学成分。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。

應用

纯化所得的高纯度DNA可直接供以下敏感的下游应用使用：
  • PCR
  • 限制性内切酶酶切
  • 克隆
  • Southern印迹
GenElute™ Plant Genomic DNA Miniprep Kit has been used:
  • in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
  • in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
  • in genomic DNA isolation from leaves

特點和優勢

  • 起始材料：不超过100 mg的植物组织
  • 预期得率：至多20 μg
  • 洗脱体积：100 - 200 μl
  • 所需时间：<40分钟
  • 是否需要RNase处理：否

原則

在1小时内，可从至多100 mg新鲜组织或10 mg冻干材料中提取出微克级的DNA。首先在液氮中研磨植物组织，在去垢剂和离液剂作用下释放出DNA。通过10分钟的沉淀操作及之后通过本试剂盒所含的滤柱进行的离心分离操作，去除蛋白质、多糖和细胞残渣。通过微量离心柱进行硅胶结合-洗涤-洗脱步骤，进一步纯化基因组DNA。纯化所得DNA长度超过20 kb。

其他說明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • C2112Column Preparation SolutionSDS

象形圖

CorrosionExclamation mark
GHS05,GHS07

訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

安全危害

EUH032

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Stefanie P Glaeser et al.
International journal of systematic and evolutionary microbiology, 63(Pt 2), 777-782 (2012-05-15)
A Gram-positive-staining, aerobic, endospore-forming bacterium, isolated from a necrotic wound of a 35-year-old man was studied in detail to determine its taxonomic position. Based on 16S rRNA gene sequence similarity comparisons, strain CCUG 53270(T) was grouped into the genus Paenibacillus
Phytohormone interplay controls proliferation of in vitro cultivated cells of Arabidopsis thaliana ethylene-insensitive mutants.
N S Stepanchenko et al.
Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 442, 46-49 (2012-03-20)
Amit Kumar Gupta et al.
Gene, 487(2), 156-159 (2011-08-11)
Isolation of intact and pure genomic DNA (gDNA) is essential for many molecular biology applications. It is difficult to isolate pure DNA from mature trees of hot and dry desert regions because of the accumulation of high level of polysaccharides
S Gadiou et al.
Virus genes, 44(2), 349-355 (2011-12-17)
A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 ×
Stefanie P Glaeser et al.
International journal of systematic and evolutionary microbiology, 63(Pt 2), 521-525 (2012-04-24)
A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain E103(T), was isolated from the skin of the medical leech Hirudo verbana. 16S rRNA gene sequence analysis showed that the isolate was closely related to species of the genus Castellaniella. Castellaniella ginsengisoli DCY36(T)
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條款

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

Plant DNA Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

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