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Sigma-Aldrich

REDExtract-N-Amp植物PCR试剂盒

sufficient for 100 extractions, sufficient for 500 amplifications

同義詞:

Plant direct PCR kit, Plant direct PCR kit with loading dye

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 100 extractions
sufficient for 500 amplifications
sufficient for 500 reactions

品質等級

特點

dNTPs included
hotstart

顏色

red

運輸包裝

wet ice

儲存溫度

−20°C

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一般說明

REDExtract-N-Amp植物PCR试剂盒提供了从植物叶片快速提取DNA以及通过PCR扩增靶标所需的所有试剂。 这是一种全新提取液,无需传统液氮冷冻植物组织、机械破碎、有机萃取、柱纯化或DNA沉淀。 该试剂盒还包括专门配制的PCR ReadyMix,可直接从提取物中扩增。 REDExtract-N-Amp PCR ReadyMix含有染料,可作为追踪染料并方便地将PCR反应产物上样到琼脂糖凝胶上进行分析。

使用标准打孔器获取0.5至0.7 cm叶片,然后在提取液中95 oC孵育10分钟,提取基因组DNA。 PCR前,将等体积的稀释液添加至提取液以中和抑制物质。 然后将一部分DNA提取物加入到含有引物和试剂盒中的REDExtract-N-Amp ReadyMix的PCR反应中。
The REDExtract-N-Amp植物PCR试剂盒能够通过直接PCR(聚合酶链反应)快速检测植物病原体。这些试剂盒提供了从植物叶片快速提取基因组DNA以及通过直接PCR扩增靶标所需的所有试剂。REDExtract-N-Amp PCR ReadyMix含有染料,可作为追踪染料并方便地将PCR反应产物上样到琼脂糖凝胶上进行分析。

應用

REDExtract-N-Amp Plant PCR Kit has been used to extract genomic DNA from plants and to perform polymerase chain reaction.

特點和優勢

  • PCR的基因组DNA只需不到 15 分钟
  • PCR ReadyMix,为提取物直接扩增而专门配制
  • 热启动抗体支持高特异性PCR扩增基因组DNA
  • 提取物稳定:在4 °C环境下稳定至少6个月
  • 一步提取PCR所需的植物基因组DNA,只需不到15分钟
  • 无需冷冻、机械破碎、有机萃取、柱纯化或液氮冷冻植物组织、机械破碎、有机提取、柱纯化或沉淀
  • 专门配制的PCR ReadyMix,与提取液配套使用
  • 热启动抗体支持高特异性PCR扩增基因组DNA
  • REDExtract-N-Amp不需要凝胶分析所需的装载缓冲液或跟踪染料
  • 符合高通量植物基因分析要求
  • 提取物稳定:在4 °C环境下稳定至少6个月

成分

试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR Reaction Mix。

其他說明

更多信息,请见www.sigma-aldrich.com/extract-n-amp

法律資訊

购买本产品包括豁免根据产品说明书中所规定的专利提起的诉讼,仅可将所购买的量用于购买者自己的内部研究。′其他专利权(例如5′核酸酶工艺的专利权)不得明示、暗示或禁止反言。 有关购买许可的更多信息,可联系许可总监(Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)获取。
根据美国专利号 5,338,671和5,587,287以及其他国家的相应专利获得抗体体外研究许可。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护: 5,789,224、5,618,711、6,127,155和美国境外的声明,对应于过期的美国专利号5,079,352。 购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何专利主张下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利,包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系许可总监(Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

象形圖

Exclamation markEnvironment

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids


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存取文件庫

Yuri Jorge Peña Ramírez et al.
Clinical and vaccine immunology : CVI, 14(6), 685-692 (2007-04-27)
The human immunodeficiency virus type 1 (HIV-1) Tat protein is considered a potential candidate vaccine antigen. In an effort to design a strategy for noninvasive vaccination against HIV-1, we developed transgenic tomatoes expressing the Tat protein. Two independent plants testing
Mark A Jones et al.
Journal of experimental botany, 58(6), 1261-1270 (2007-02-16)
Reactive oxygen species (ROS) production by an NADPH oxidase (NOX) encoded by AtrbohC/RHD2 is required for root hair growth in Arabidopsis thaliana. ROP (RHO of plants) GTPases are also required for normal root hair growth and have been proposed to
Ralf G Kynast et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(26), 9921-9926 (2004-06-16)
We have developed from crosses of oat (Avena sativa L.) and maize (Zea mays L.) 50 fertile lines that are disomic additions of individual maize chromosomes 1-9 and chromosome 10 as a short-arm telosome. The whole chromosome 10 addition is
Avinash C Srivastava et al.
Plant physiology, 148(1), 200-211 (2008-07-25)
AtSUC2 (At1g22710) encodes a phloem-localized sucrose (Suc)/H(+) symporter necessary for efficient Suc transport from source tissues to sink tissues in Arabidopsis (Arabidopsis thaliana). AtSUC2 is highly expressed in the collection phloem of mature leaves, and its function in phloem loading
Anna Stöger et al.
Journal of microbiological methods, 58(2), 281-284 (2004-07-06)
Angular leaf spot is a bacterial disease caused by Xanthomonas fragariae. It has become a serious disease in the USA and Europe in recent years. Several detection procedures are described for this plant pathogen. However, they are either too time-consuming

文章

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

條款

The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves.

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