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XNAT2R

Sigma-Aldrich

Extract-N-Amp ™ 组织 PCR 试剂盒

sufficient for 1000 extractions, sufficient for 1000 amplifications

同義詞:

Tissue direct PCR kit

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 1000 amplifications
 sufficient for 1000 extractions
 sufficient for 1000 reactions

特點

dNTPs included
hotstart

技術

PCR: suitable

顏色

colorless

運輸包裝

wet ice

儲存溫度

−20°C

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相關類別

一般說明

Extract-N-Amp™组织PCR试剂盒为从各种细胞和组织中快速提取DNA并通过直接PCR（聚合酶链反应）扩增靶标提供了所有必需试剂。这种新型提取方法无需长时间的酶消化或均质化处理。该试剂盒还包括专门配制的热启动PCR ReadyMix™，可直接从提取物中扩增。

應用

Extract-N-Amp™ tissue PCR kit has been used for DNA extraction and polymerase chain reaction (PCR).

特點和優勢

  • 快速 – 在15 分钟内快速提取用于 PCR 的基因组 DNA
  • 方便-不需要漫长的酶消化或柱纯化
  • 实用 – 基因分型用快速基因组 DNA 分离的完美选择
  • 灵活 – 可提供适合鼠尾、毛发、动物组织、唾液和口腔拭子的操作方案
  • 特异性-用于基因组 DNA 高特异性 PCR 扩增的热启动抗体
  • 提取物在4oC下可稳定保存至少 6 个月。

成分

试剂盒含有Tissue Preparation Solution、Extraction Solution和Extract-N-Amp PCR Reaction Mix。

原則

该试剂盒包含从小鼠尾、毛发、动物组织、唾液和口腔拭子中提取和扩增基因组DNA的经验证实验方案。在常规操作步骤中，基因组DNA提取自在室温下的组织制备溶液和提取溶液中孵育10分钟的样本。将该样本加热至95 °C并持续3分钟，然后混入第三种溶液以便在PCR反应前中和抑制物。随后将一部分DNA提取物加入到含有引物和Extract-N-Amp PCR ReadyMix（包含在试剂盒内）的PCR反应物中。

其他說明

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
This kit includes a clear solution of PCR readymix. To order the REDExtract-N-Amp Tissue PCR Kits with the inert tracking and loading dye in the PCR readymix, please order XNAT-1000RXN"

法律資訊

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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訊號詞

Danger

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Stefano Boi et al.
Virology, 499, 136-143 (2016-09-23)
We have demonstrated in a mouse model that infection with a retrovirus can lead not only to the generation of recombinants between exogenous and endogenous gammaretrovirus, but also to the mobilization of endogenous proviruses by pseudotyping entire polytropic proviral transcripts
Sergio Y Alcoser et al.
BMC biotechnology, 11, 124-124 (2011-12-20)
Xenograft samples used to test anti-cancer drug efficacies and toxicities in vivo contain an unknown mix of mouse and human cells. Evaluation of drug activity can be confounded by samples containing large amounts of contaminating mouse tissue. We have developed
Cindy Y Kok et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 21(10), 1823-1831 (2013-07-03)
Viral vectors based on adeno-associated virus (AAV) are showing exciting promise in gene therapy trials targeting the adult liver. A major challenge in extending this promise to the pediatric liver is the loss of episomal vector genomes that accompanies hepatocellular
Graziella Madeo et al.
Movement disorders : official journal of the Movement Disorder Society, 29(1), 41-53 (2013-10-30)
Homozygous or compound heterozygous mutations in the phosphatase and tensin homolog-induced putative kinase 1 (PINK1) gene are causative of autosomal recessive, early onset Parkinson's disease. Single heterozygous mutations have been detected repeatedly both in a subset of patients and in
Xiaodong Sun et al.
Cell cycle (Georgetown, Tex.), 12(7), 1133-1141 (2013-03-09)
Parkin, an E3 ubiquitin ligase well known for its role in the pathogenesis of juvenile Parkinson disease, has been considered as a candidate tumor suppressor in certain types of cancer. It remains unknown whether parkin is involved in the development
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文章

Extract-N-Amp™ Tissue Feature Article

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Extract-N-Amp™ Tissue Feature Article

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

條款

Extract-N-Amp™ Tissue PCR Kit Protocol

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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