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D7440

Sigma-Aldrich

JumpStart™ Taq ReadyMix™预混液（定量PCR用）

For probe-based real-time PCR

同義詞:

hot start DNA polymerase, hot start PCR, hot start master mix, qPCR, real time quantitative PCR

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

品質等級

200

形狀

liquid

用途

sufficient for 100 reactions
 sufficient for 20 reactions
 sufficient for 400 reactions

特點

Multiplex PCR
dNTPs included
hotstart

濃度

2.5 units/reaction (50 μL reaction volume)

技術

qPCR: suitable

顏色

colorless

輸入

purified DNA

應用

agriculture

相容性

Bio-Rad MyiQ ( )
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

檢測方法

probe-based

運輸包裝

wet ice

儲存溫度

−20°C

相關類別

一般說明

JumpStart™ Taq ReadyMix™是一种即用型2X预混液，其中含有JumpStart™ Taq DNA聚合酶、99％纯dNTP、反应缓冲液和JumpStart™抗体。它将我们用于热启动PCR的JumpStart ™Taq抗体的高效表现和易用型反应混合物的方便性结合在一起。由于它没有添加染料，因此是依赖于荧光探针的高通量定量PCR方法的理想解决方案。Taq DNA聚合酶是一种抗体灭活的热启动酶。一旦反应温度达到70°C，DNA聚合酶-抗体复合物随即解离，Taq DNA聚合酶活性得以恢复。相对于手动热启动技术，这种抗体-酶复合物可轻松简便地准备，同时可降低污染风险。

應用

JumpStart™ Taq ReadyMix™预混液（用于定量PCR）已用于：

  • 定量聚合酶链式反应(qPCR)扩增HIV-1 RNA（逆转录）和DNA（使用探针）
  • 在监测甘草在Treg细胞分化和功能中的活性研究中，用于通过2步法RT-qPCR分析定量逆转录RNA
  • 作为反应混合物组分，用于检测并扩增沙门氏菌和扩增内标(IAC) pUC 19质粒DNA
  • 作为反应混合物组分，用于通过定量聚合酶链式反应(qPCR)检测艰难梭菌
  • 用于逆转录的ß-arrestin-1 (ARRB1)和β-arrestin-2 (ARRB2) mRNA的实时定量PCR

特點和優勢

  • 终极便利：ReadyMix包含QPCR所有必需的成分，仅需将荧光检测试剂、引物和模板加入即可
  • 特异性更高&目标产量更大JumpStart™ Taq可防止非特异性扩增并提高靶标的产量
  • 最大的灵活性：适用于一系列检测试剂，包括分子探针和双链结合染料，如SYBR® Green I
  • 适用于板/管配置的实时热循环仪或毛细管类仪器。

包裝

该ReadyMix产品包含单独包装的Sigma参比染料（定量PCR用），用于标准化反应数据。 染料的最大激发波长为586 nm，最大发射波长为605 nm。 ROX参考染料仪器设定符合定量PCR参考染料测量需要。 另提供一管25 mM MgCl2，以便于优化qPCR反应。

默认反应体积为50 μL

20RXN 包装为 1 X 500 μL
100RXN 包装为 1 X 2.5 mL
400RXN 包装为 1 X 10 mL

其他說明

JumpStart Taq ReadyMix（定量PCR用）结合热启动酶与即用型预混液优势，适用于高通量定量PCR(qPCR)。 它不含检测试剂的配方使其适合与多种试剂一起使用，包括双标记探针、分子信标或双链结合染料，如SYBR Green I。

原則

ReadyMix预混液包括JumpStart Taq DNA聚合酶、99％纯脱氧核苷酸、以及优化的2倍浓缩缓冲液。 在配置反应体系时，可将25 μL的ReadyMix加入到引物、模板、检测试剂和水中以达到50 μL的总反应体积。配置可在室温进行，因为JumpStart Taq抗体可使Taq DNA聚合酶处于失活状态。 在第一个变性循环中，抗体会从酶上解离，从而使活性完全恢复。 无需进行特殊的准备或修改实验操作。

單位定義

在 74℃ 下一个单位在 30 min 内将 10 nmol 的总 dNTP 掺入到酸可沉淀的 DNA 中。

法律資訊

研究人员需要(i)同时购买Authorized 5′ Nuclease Core Kit授权核酸酶核心试剂盒和Licensed Probe许可探针，(ii)只购买1种Licensed 5′ Nuclease Kit许可核酸酶试剂盒或(iii)向Applied Biosystems公司购买许可权，才能获得许可进行专利所有的5′ Nuclease Process核酸酶实验流程。

本品属于Authorized 5′ Nuclease Core Kit授权核酸酶核心试剂盒。本产品的使用受到下列美国专利No. 5,210,015和5,487,972对应的美国境外的一项或多项专利声明保护。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。单独购买Licensed Probe许可探针将获得美国专利及其对应的境外专利声明权利： 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375（仅1-12项声明），以及美国专利No. 6,214,979对应的美国境外专利。购买者无权使用任何其他专利权利要求，无权进行任何形式的商业服务，包括但不限于以收费或其他商业考虑的形式对购买者′的活动结果进行报道，该产品没有明示或暗示地以禁止反言或其他任何方式,授予过任何知识产权许可。  本品仅供研究使用。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • P2893JumpStart™ Taq ReadyMix™, Complete optimized reagent for hot-start PCR at 2X concentration 2 XSDS
  • M878725 mM MgCl2 1.5 μLSDS
  • R4526Reference Dye for Quantitative PCR, 100 ×, solution 100 XSDS

象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Cawas B Engineer et al.
Plant physiology, 143(1), 236-250 (2006-11-07)
Nitrogen is an essential macronutrient for plant growth and survival. Here, the temporal and spatial sensing of nitrogen starvation is analyzed in Arabidopsis (Arabidopsis thaliana). The promoter for the high-affinity ammonium transporter, AtAmt1.1, is shown to be a valid indicator
Benjamin B Gelman et al.
Journal of acquired immune deficiency syndromes (1999), 62(5), 487-495 (2012-12-18)
Replicating HIV-1 in the brain is present in HIV encephalitis (HIVE) and microglial nodule encephalitis (MGNE) and is putatively linked with HIV-associated neurocognitive disorders (HAND). A cliniconeurovirological correlation was conducted to elucidate the relationship between brain viral load and clinical
Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells.
Wang, Q., et al.
Cell Research (2011)
Andrew J Levine et al.
Journal of neurovirology, 22(3), 366-375 (2015-12-23)
HIV infection leads to age-related conditions in relatively young persons. HIV-associated neurocognitive disorders (HAND) are considered among the most prevalent of these conditions. To study the mechanisms underlying this disorder, researchers need an accurate method for measuring biological aging. Here
Juan Li et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 32(8), 4096-4106 (2018-02-28)
β-Arrestins (β-arrestin-1 and -2) are multifunctional proteins that play important roles in the regulation of inflammation and cell survival that need to be tightly controlled; however, the mechanism that underlies their gene expression is largely unclear. Here, we demonstrate that
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文章

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

Probe-based qPCR

Probe based QPCR utilizes a fluorescent–labeled target-specific probe resulting in increased specificity and sensitivity. Additionally, a variety of fluorescent dyes are available so that multiple primers can be used to simultaneously amplify many sequences.

Using Probe-Based Quantitative PCR (Qpcr) to Measure Gene-Level Expression

Quantitative PCR (qPCR) provides information about gene expression, gene amplification or loss, and small alterations. qPCR is often used to investigate tumor biology and to discover the genetic and epigenetic causes of cancer

Polymerase Chain Reaction

The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension

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