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D6442

Sigma-Aldrich

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR

Ready-to-use 2x mix for qPCR with ROX

同義詞:

Taq polymerase mix

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55

形狀

liquid

用途

sufficient for 20 reactions
 sufficient for 2000 reactions
 sufficient for 400 reactions

特點

dNTPs included
hotstart

濃度

2.5 units/reaction (50 μL reaction volume)

技術

qPCR: suitable

顏色

colorless

輸入

purified DNA

應用

agriculture

相容性

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

檢測方法

probe-based

運輸包裝

wet ice

儲存溫度

−20°C

相關類別

一般說明

JumpStart™ Taq ReadyMix™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.
To prepare a typical qPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL. Reaction volumes can be scaled down, if desired.

應用

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR has been used:
  • For quantitative polymerase chain reaction (qPCR) of cDNA template
  • As a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytes
  • For qPCR amplifications using SYBR
  • For multiplex PCR
  • For reduction of primer dimers

特點和優勢

  • JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • When performing large number of PCR reactions, JumpStart™ Taq ReadyMix™ reduces preparation time and the risk of contamination from multiple pipetting steps
  • This master mix allows consistent batch-to-batch and reaction-to-reaction performance
  • Internal Reference Dye is provided for reaction normalization

包裝

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

數量

Use 2.5 units per reaction.

法律資訊

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

相關產品

產品號碼
描述
訂價

W1754

水, PCR Reagent

訊號詞

Danger

危險分類

Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Eye Dam. 1 - Ox. Liq. 3 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

5.1B - Oxidizing hazardous materials

分析證明 (COA)

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Evrim Komurcu-Bayrak et al.
Clinica chimica acta; international journal of clinical chemistry, 383(1-2), 110-115 (2007-06-09)
We evaluated the relationship of the lipoprotein lipase (LPL) S447X variant with serum lipid levels and the metabolic syndrome (MS) in the Turkish Adult Risk Factor (TARF) study. This is the first study examining this LPL variant in the Turkish
Irene B Hanning et al.
Methods in molecular biology (Clifton, N.J.), 733, 159-170 (2011-03-25)
Next-generation sequencing (NGS) is a powerful tool that can be utilized to profile and compare microbial populations. By amplifying a target gene present in all bacteria and subsequently sequencing amplicons, the bacteria genera present in the populations can be identified
Edouard Jurkevitch
Current protocols in microbiology, Chapter 7, Unit7B-Unit7B (2012-08-10)
Bdellovibrio and like organisms (BALOs) are obligate predators of Gram-negative bacteria. BALOs are isolated as plaques growing at the expense of their prey and are cultivated as two-member cultures. The growth cycle is composed of an extracellular attack phase and
Adrien Chabot et al.
Genetics, 176(4), 2069-2076 (2007-06-15)
Most phenotypic differences between human and chimpanzee are likely to result from differences in gene regulation, rather than changes to protein-coding regions. To date, however, only a handful of human-chimpanzee nucleotide differences leading to changes in gene regulation have been
Bilge Ozsait et al.
Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology, 8(5), 324-330 (2008-10-14)
In this study, our aim was to investigate the association of cholesterol ester transfer protein (CETP) TaqIB polymorphism with the likelihood of metabolic syndrome (MetS). Study was designed as a cross-sectional analysis of the Turkish Adult Risk Factor follow-up study.
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