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47608

Sigma-Aldrich

Formaldehyde solution

for molecular biology, BioReagent, ≥36.0% in H2O (T)

Synonym(s):

Formic aldehyde, Formol, Formalin

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About This Item

Linear Formula:
HCHO
CAS Number:
50-00-0
Molecular Weight:
30.03
Beilstein/REAXYS Number:
1209228
MDL number:
MFCD00003274
UNSPSC Code:
12352114
PubChem Substance ID:
329757328
NACRES:
NA.52

grade

for molecular biology

Quality Level

100

vapor density

1.03 (vs air)

vapor pressure

52 mmHg ( 37 °C)

product line

BioReagent

form

liquid

autoignition temp.

572 °F

contains

~10% methanol as stabilizer

concentration

≥36.0% in H2O (T)
36.0-38.0% (aldehyde method)

technique(s)

electrophoresis: suitable

impurities

≤0.03% free acid (as HCOOH)

ign. residue

≤0.05% (as SO4)

pH

2.8-4.0

density

1.09 g/mL at 20 °C
1.09 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Zn: ≤1 mg/mL

λ

2 M in H2O

UV absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

suitability

in accordance for electrophoresis of RNA in agarose gels

storage temp.

room temp

SMILES string

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

Application

Formaldehyde solution is suitable for:
  • denaturation and separation of RNA molecules
  • anti-bacterial treatment
  • fixation of cells
Formaldehydesolution has been used:
  • in the fixation of human spermatogonial stem cell transplantation (SSCT).
  • to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
  • in fixing zebra fish larvae for neutrophil staining.

Caution

may contain precipitate (paraformaldehyde)

Principle

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

133.0 °F - closed cup

flash_point_c

56.11 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Jin Qian et al.
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Interferon-induced transmembrane (IFITM) proteins inhibit the entry of a large number of viruses. Not surprisingly, many viruses are refractory to this inhibition. In this study, we report that different strains of HIV and SIV are inhibited by human IFITM proteins
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Epstein-Barr virus (EBV) causes human lymphoid malignancies, and the EBV product latent membrane protein 1 (LMP1) has been identified as an oncogene in epithelial carcinomas such as nasopharyngeal carcinoma (NPC). EBV can epigenetically reprogram lymphocyte-specific processes and induce cell immortalization.
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The production of ribosomes is ubiquitous and fundamental to life. As such, it is surprising that defects in ribosome biogenesis underlie a growing number of symptomatically distinct inherited disorders, collectively called ribosomopathies. We previously determined that the nucleolar protein, NOL11
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