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Key Documents
V900503
Peroxidase from horseradish
Vetec™, reagent grade
Synonim(y):
Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
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About This Item
Polecane produkty
klasa czystości
reagent grade
linia produktu
Vetec™
Postać
powder
aktywność właściwa
≥250 units/mg solid
masa cząsteczkowa
~44 kDa
temp. przechowywania
2-8°C
InChI
1S/H2O3/c1-3-2/h1-2H
Klucz InChI
JSPLKZUTYZBBKA-UHFFFAOYSA-N
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Opis ogólny
Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
Definicja jednostki
One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Komentarz do analizy
The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.
Inne uwagi
View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.
Informacje prawne
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
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