P2088

Peroxidase from horseradish

Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)

• Synonim(y): Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
• Numer CAS: 9003-99-0
• Numer EC enzymu: 1.11.1.7 (BRENDA, IUBMB)
• Numer WE: 232-668-6
• Numer MDL: MFCD00071339
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: horseradish
• Poziom jakości: 300
• Formularz: essentially salt-free, lyophilized powder
• aktywność właściwa: 200-300 units/mg solid (using pyrogallol)
• masa cząsteczkowa: ~44 kDa
• stosunek absorbancji: RZ 2.6-3.4
• temp. przechowywania: 2-8°C
• ciąg SMILES: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• Klucz InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Opis

Opis ogólny

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca²⁺ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Zastosowanie

Peroxidase from horseradish has been used in a study with dehydrogenative polymers (DHP) to investigate the monolignol coupling mechanism. It has also been used to help develop a colorimetric method for microRNA (miRNA) detection by creating a horseradish peroxidase-mimicking DNAzyme.

Działania biochem./fizjol.

Horseradish peroxidase has been shown to catalyze the polymerization of various substances including acetaminophen and cardanol.

Przestroga

Maintains activity at low pH and higher temperature.

Definicja jednostki

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Uwaga dotycząca przygotowania

Stabilized by chemical protection of the primary amines.

Komentarz do analizy

The RZ (Reinheitszahl) is the absorbance ratio A₄₀₃/A₂₇₅ determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Inne uwagi

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)