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Key Documents
C5896
Choline Oxidase from Alcaligenes sp.
lyophilized powder, ≥10 units/mg solid
Synonim(y):
Choline: oxygen 1-oxidoreductase
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About This Item
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lyophilized powder
Poziom jakości
aktywność właściwa
≥10 units/mg solid
masa cząsteczkowa
~95 kDa
metody
cell based assay: suitable
przydatność
suitable for assay of choline
suitable for molecular biology
temp. przechowywania
−20°C
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Zastosowanie
Choline oxidase from Alcaligenes sp. has been used:
- in a study to investigate a choline biosensor constructed with a chitinous membrane and its application in measuring cholinesterase inhibitory activities
- has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.
- as a component of phosphate buffer saline (PBS) buffer for measurement of choline acetyltransferase (ChAT) activity in brain tissue homogenates
- to measure the choline generated by autotaxin (ATX) by ATX activity assay and phospholipase-D (PLD) by PLD activity assay
Działania biochem./fizjol.
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as the intermediate and molecular oxygen as th eprimary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the study of the reaction mechanism for the conversion of choline to the aldehyde intermediate, & of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approx. 72,000 Da (gel filtration) or 66,000 Da (SDS gel electrophoresis).
Właściwości fizyczne
Isoelectric point:4.1 ± 0.1
Michaelis constants:2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde)
Structure:One mol of FAD is covalently bound to mol of the enzyme
Inhibitors:p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+
Optimum pH:8.0 – 8.5
Optimum temp:40 – 45°C
pH Stability:pH 7.0 – 9.0 (30°C, 2hr)
Thermal stability:Below 37°C (pH 7.5, 10min)
Michaelis constants:2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde)
Structure:One mol of FAD is covalently bound to mol of the enzyme
Inhibitors:p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+
Optimum pH:8.0 – 8.5
Optimum temp:40 – 45°C
pH Stability:pH 7.0 – 9.0 (30°C, 2hr)
Thermal stability:Below 37°C (pH 7.5, 10min)
Definicja jednostki
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
Certyfikaty analizy (CoA)
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