P0065

Phospholipase D from Streptomyces chromofuscus

≥50,000 units/mL, buffered aqueous glycerol solution

• Synonim(y): Lecithinase D, Phosphatidylcholine phosphatidohydrolase
• Numer CAS: 9001-87-0
• Numer EC enzymu: 3.1.4.4 (BRENDA, IUBMB)
• Numer WE: 232-639-8
• Numer MDL: MFCD00131897
• Kod UNSPSC: 41106300
• NACRES: NA.77

Właściwości

• Postać: buffered aqueous glycerol solution
• aktywność właściwa: ≥50,000 units/mL
• masa cząsteczkowa: ~60 kDa
• Warunki transportu: dry ice
• temp. przechowywania: −70°C

Opis

Opis ogólny

Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.

Zastosowanie

Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).

Działania biochem./fizjol.

Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.

Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Definicja jednostki

One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 30 °C.

Postać fizyczna

Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)