77332

Peroxidase from horseradish

lyophilized, powder, ~150 U/mg

• Synonim(y): (2R,3S,6S,7R,8R)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl 3-methylbutanoate, Antimycin A, Horseradish peroxidase
• Numer CAS: 9003-99-0
• Numer EC enzymu: 1.11.1.7 (BRENDA, IUBMB)
• Numer WE: 232-668-6
• Numer MDL: MFCD00071339
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: horseradish
• Poziom jakości: 100
• Postać: powder
• jakość: lyophilized
• aktywność właściwa: ~150 U/mg
• masa cząsteczkowa: M_r ~40000
• rozpuszczalność: 50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• InChI: 1S/H2O3/c1-3-2/h1-2H
• Klucz InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Opis

Opis ogólny

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.

Zastosowanie

Peroxidase from horseradish has been used:

• for isolation of N-Linked glycans
• as a constituent of D-amino acid oxidase (DAAO) buffer
• to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.

Definicja jednostki

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Inne uwagi

Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)