SRE0082

Peroxidase from horseradish

• Synonim(y): Peroxidase from horseradish, Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
• Numer CAS: 9003-99-0
• Numer EC enzymu: 1.11.1.7 (BRENDA, IUBMB)
• Numer WE: 232-668-6
• Numer MDL: MFCD00071339
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• opis: For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US.
• Poziom jakości: 400
• aktywność właściwa: ≥250 units/mg solid (using pyrogallol)
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C
• InChI: 1S/H2O3/c1-3-2/h1-2H
• Klucz InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Opis

Opis ogólny

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca²⁺ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Zastosowanie

Peroxidase from horseradish has been used in western blotting.

Definicja jednostki

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Komentarz do analizy

The RZ (Reinheitszahl) is the absorbance ratio A₄₀₃/A₂₇₅ determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Inne uwagi

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1