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Merck

T1005

Sigma-Aldrich

Trypsin from bovine pancreas

Type XI, lyophilized powder, ≥6,000 BAEE units/mg protein

Synonim(y):

Serine Protease 1

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About This Item

Numer CAS:
Numer EC enzymu:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352204

pochodzenie biologiczne

bovine pancreas

Poziom jakości

typ

Type XI

Postać

lyophilized powder

aktywność właściwa

≥6,000 BAEE units/mg protein

masa cząsteczkowa

23.8 kDa

skład

protein, 90-100%

zanieczyszczenia

salt, essentially free

rozpuszczalność

hydrochloric acid: soluble 1 mM, clear

obecność zanieczyszczeń

Chymotrypsin ≤0.1 BTEE units/mg protein

temp. przechowywania

−20°C

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Zastosowanie

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Trypsin from bovine pancreas has been used:-
  • For Trypsinization, for eliminating the membrane bound alloantigen.
  • For incubating 20-kD amylase-binding component, to evaluate its Chemical nature.
  • To prepare lactoferrin degradation products for evaluating its antiadipogenic activity.

Działania biochem./fizjol.

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Komponenty

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Przestroga

Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.

Definicja jednostki

One BAEE unit will produce a A253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as a substrate.

Uwaga dotycząca przygotowania

This product is from pancreas sourced from New Zealand. It is soluble in 1 mM HCl at 1 mg/mL.
This page may contain text that has been machine translated.

Piktogramy

Exclamation markHealth hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

H Wagner et al.
The Journal of experimental medicine, 148(6), 1523-1538 (1978-12-01)
Secondary murine cytotoxic T lymphocyte responses from alloantigen-primed T cells can be induced in vitro by apparently unrelated regimens, such as addition of either concanavalin A (Con A), conditioned medium from Con A stimulated lymphocyte cultures, conditioned medium from secondary
F A Scannapieco et al.
Infection and immunity, 60(11), 4726-4733 (1992-11-01)
The goal of the present study was to begin characterizing the amylase-binding component(s) on the surface of Streptococcus gordonii G9B. Alkali extracts but not phenol-water extracts of this bacterium inhibited 125I-amylase binding to S. gordonii G9B. To identify the bacterial
Tomoji Ono et al.
The British journal of nutrition, 105(2), 200-211 (2010-09-22)
Lactoferrin (LF) is a multifunctional glycoprotein in mammalian milk. In a previous report, we showed that enteric-coated bovine LF tablets can decrease visceral fat accumulation, hypothesising that the enteric coating is critical to the functional peptides reaching the visceral fat
Derek Toms et al.
Frontiers in bioengineering and biotechnology, 8, 534-534 (2020-06-26)
We have developed an accessible software tool (receptoR) to predict potentially active signaling pathways in one or more cell type(s) of interest from publicly available transcriptome data. As proof-of-concept, we applied it to mouse photoreceptors, yielding the previously untested hypothesis
Kenneth N Grisé et al.
Stem cell research & therapy, 12(1), 83-83 (2021-01-27)
Adult mammalian retinal stem cells (RSCs) readily proliferate, self-renew, and generate progeny that differentiate into all retinal cell types in vitro. RSC-derived progeny can be induced to differentiate into photoreceptors, making them a potential source for retinal cell transplant therapies.

Protokoły

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

Ciągła spektrofotometryczna metoda oznaczania szybkości przy użyciu substratu BAEE mierzy aktywność trypsyny, niezbędną do charakterystyki enzymu.

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