650279

Trypsin, Porcine, MS Grade

• Synonim(y): Porcine Trypsin
• Kod UNSPSC: 12352202
• NACRES: NA.54

Właściwości

• klasa czystości: Proteomics Grade
• Poziom jakości: 100
• Postać: lyophilized
• aktywność właściwa: ≥10,000 U/mg
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• przydatność: suitable for mass spectrometry
• obecność zanieczyszczeń: Chymostrypsin <3 BTEE units/mg solid
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin, and 1 ml of resuspension buffer.

A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry.

Ostrzeżenie

Toxicity: Harmful (C)

Definicja jednostki

One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.

One BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8

Rekonstytucja

Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.

Inne uwagi

Henzel, W.J., et al. 1993. PNAS90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta492, 316..

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H334,H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 10 - Combustible liquids