T7575
Trypsin Singles, Proteomics Grade
Synonim(y):
Proteomics grade, Trypsin, TPCK treated
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About This Item
Kod UNSPSC:
12352204
NACRES:
NA.56
Polecane produkty
klasa czystości
Proteomics Grade
Postać
ready-to-use solution
aktywność właściwa
≥10,000 BAEE units/mg protein
masa cząsteczkowa
average mol wt 23.29 kDa
optymalne pH
~8.0
temp. przechowywania
2-8°C
Powiązane kategorie
Opis ogólny
Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.
Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.
Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.
Zastosowanie
Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry). It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.
Cechy i korzyści
- Convenient, single-use 1 μg size
- Eliminates repetitive pipetting
- Reductively methylated to minimize autolytic activity
- TPCK treated to quench chymotryptic activity
- Extensively purified
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Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
3 - Flammable liquids
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