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Kluczowe dokumenty
SML1665
Nocodazole
≥98% (HPLC), DMSO solution, microtubule inhibitor
Synonim(y):
Methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate, Nocidazole, Nocodazolum, Oncodazole, R 17934
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About This Item
Wzór empiryczny (zapis Hilla):
C14H11N3O3S
Numer CAS:
Masa cząsteczkowa:
301.32
Kod UNSPSC:
12352200
NACRES:
NA.77
Polecane produkty
product name
Nocodazole Ready Made Solution, 5 mg/mL, DMSO solution
pochodzenie biologiczne
synthetic
Poziom jakości
Postać
DMSO solution
stężenie
5 mg/mL
Warunki transportu
dry ice
temp. przechowywania
−20°C
InChI
1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
Klucz InChI
KYRVNWMVYQXFEU-UHFFFAOYSA-N
Działania biochem./fizjol.
Nocodazole is an anticancer drug that has been shown to interfere with the structure and function of microtubules in interphase and mitotic cells. Malignant cells may be more susceptible to the antimicrotubular effect of nocodazole than nonmalignant cells. Mammalian cells cultured in vitro can be treated with 0.04-10 μg/mL doses for cell synchronization experiments. Prolonged arrest of cells in mitosis due to nocodazole treatment typically results in cell death by apoptosis. Higher concentrations could not be used because of insolubility. High specificity of action may explain low toxicity to bone marrow cells and lack of neurotoxicity. Nocodazole is thought to bind directly to tubulin causing conformational changes resulting in increased exposure of some sulfhydryl and possibly tyrosine residues. Nocodazole′s apparent synergism with cytosine arabinofuranoside has been demonstrated on L1210 leukemic cells.
Uwaga dotycząca przygotowania
Nocodazole forms precipitates in frozen solution, therefore sonication should be applied after thaw to homogenize the solution. It is recommended to sonicate for 1-3 minutes post thawing to remove precipitates that might appear due to freezing.
For cell culture work, dilute Nocodazole ready-made solution (5 mg/mL DMSO) in the culture media, diluted solution is stable for at least 7 days in biological. Mammalian cells cultured in vitro can be treated with 0.04-10 μg/mL doses.
For cell culture work, dilute Nocodazole ready-made solution (5 mg/mL DMSO) in the culture media, diluted solution is stable for at least 7 days in biological. Mammalian cells cultured in vitro can be treated with 0.04-10 μg/mL doses.
Przechowywanie i stabilność
The product is stable for 2 years at -20C.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
188.6 °F - closed cup
Temperatura zapłonu (°C)
87 °C - closed cup
Certyfikaty analizy (CoA)
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