NA9604

GenElute^™ HP 96-Well Plasmid Miniprep Kit

sufficient for 4 96-well plate purifications

• Synonim(y): 96-dołkowy zestaw do ekstrakcji plazmidów, 96-dołkowy zestaw do izolacji plazmidów, 96-dołkowy zestaw do oczyszczania plazmidów, Zestaw do minipreparacji o wysokiej przepustowości, Zestaw plazmidowy o wysokiej przepustowości, zestaw do ekstrakcji plazmidów, zestaw do izolacji plazmidów, zestaw do oczyszczania plazmidów
• Kod UNSPSC: 41105501
• NACRES: NA.52

Właściwości

• zastosowanie: sufficient for 4 96-well plate purifications
• Poziom jakości: 200
• metody: DNA purification: suitable
• temp. przechowywania: 15-25°C

Opis

Opis ogólny

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure. Bacterial cells are harvested by centrifugation and resuspended in Resuspension Solution containing RNase. The cells are then lysed and neutralized followed by the addition of Binding Solution. The prepared lysate is then transferred to a Sigma HP Filter Plate assembled in a vacuum manifold where it is filtered and collected into a deep well plate. The vacuum manifold is rearranged and the filtered lysate is transferred to the Sigma HP Binding Plate. The plasmid DNA is captured onto the silica based membrane and impurities are removed by successive wash steps. Finally, the binding plate is dried and the plasmid DNA is eluted in Elution Solution.

The GenElute HP 96 Well Plasmid Miniprep Kit offers a simple, rapid, costeffective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.

The GenElute HP 96-Well Plasmid Miniprep Kit offers a simple, rapid, cost effective solution for high-throughput purification of plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a vacuum format, up to 10 μg of high copy plasmid DNA per well can be recovered from 1.3 mL of culture in less than 50 minutes. Actual yields and the optimum volume of culture depend on the plasmid and culture medium used.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

Zastosowanie

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

Cechy i korzyści

• Up to 10 μg of high-copy plasmid DNA per well
• Flexibility of both manual and automated formats
• Optimized for production of virus titers from MISSION shRNA plasmid vectors
• No phenol/chloroform extraction or alcohol precipitation required

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02,GHS08,GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H226,H290,H302,H314,H334,H336
• Zwroty wskazujące środki ostrożności: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
• Organy docelowe: Central nervous system
• Kod klasy składowania: 3 - Flammable liquids
• Temperatura zapłonu (°F): 77.0 °F
• Temperatura zapłonu (°C): 25 °C