NA0160

GenElute^™ HP Plasmid Miniprep Kit

sufficient for 350 preparations

• Synonim(y): GenElute^™ HP Plasmid Kit, HP miniprep kit, fast miniprep kit, fast plasmid isolation kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation kit, Gen Elute
• Kod UNSPSC: 41105501
• NACRES: NA.52

Właściwości

• zastosowanie: sufficient for 350 preparations
• Poziom jakości: 200
• charakterystyka ekologicznej alternatywy: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• metody: DNA extraction: suitable
• kategoria ekologicznej alternatywy: Aligned,
• temp. przechowywania: 15-25°C

Opis

Opis ogólny

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.

The GenElute^™ HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Zastosowanie

The recovered plasmid DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transcription, and sequencing.

Cechy i korzyści

• Purified plasmid DNA in less than 30 minutes
• Up to 25 μg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• No phenol/chloroform extraction or alcohol precipitation required

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS07,GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H290 - H302 - H314 - H334 - H336
• Zwroty wskazujące środki ostrożności: P234 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
• Organy docelowe: Central nervous system
• Kod klasy składowania: 8A - Combustible, corrosive hazardous materials
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable