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Key Documents
PLN350
GenElute™ Plasmid Miniprep Kit
sufficient for 350 purifications
Synonim(y):
GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
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About This Item
Kod UNSPSC:
41105501
NACRES:
NA.52
Polecane produkty
zastosowanie
sufficient for 350 purifications
Poziom jakości
charakterystyka ekologicznej alternatywy
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
metody
DNA purification: suitable
kategoria ekologicznej alternatywy
temp. przechowywania
15-25°C
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Opis ogólny
The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Zastosowanie
The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.
The kit has been used for the purification of plasmid DNA of Dirofilaria repens.
Cechy i korzyści
- Purified plasmid DNA in less than 30 minutes
- Purify up to 15 μg of plasmid DNA
- No detectable genomic DNA or RNA contamination
- No phenol/chloroform extraction or alcohol precipitation required
Inne uwagi
For additional information, please see www.sigma-aldrich.com/genelutehp.
Informacje prawne
GenElute is a trademark of Sigma-Aldrich Co. LLC
This page may contain text that has been machine translated.
produkt powiązany
Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organy docelowe
Central nervous system
Kod klasy składowania
3 - Flammable liquids
Certyfikaty analizy (CoA)
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