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NA1020

Sigma-Aldrich

GenElute™ PCR Clean-Up Kit

sufficient for 70 purifications

Synonim(y):

Gen Elute, PCR Purification

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About This Item

Kod UNSPSC:
41106300
NACRES:
NA.52

zastosowanie

sufficient for 70 purifications

Poziom jakości

200

metody

DNA purification: suitable

temp. przechowywania

15-25°C

Opis ogólny

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Zastosowanie

GenElute™ PCR Clean-Up Kit has been used for rapid purification of single-stranded or double-stranded PCR amplification products (100bp to 10kb) from other components in the reaction.
Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.

Cechy i korzyści

  • Purifies up to 100 μl or 10 μg of PCR amplified DNA in 8 minutes
  • Recovers up to 95% of PCR products between 100 bp and 10 kb
  • Removes over 99% of primers and other components
  • Eliminates the need to remove mineral oil by organic extraction
  • 40% more purification preps supplied than market leader

Inne uwagi

The GenElute PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reactions, such as excess primers, nucleotides, DNA polymerase, oil and salts. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.

Zasada

The GenElute PCR Clean-Up Kit combines the advantages of silica binding with a microspin format. The DNA is bound on a silica membrane within the spin column. The bound DNA is washed and the clean, concentrated DNA is eluted in the buffer of choice. Each column can purify up to 100 μL or 10 μg of PCR amplified DNA and recover up to 95% of PCR products between 100 bp and 10 kb. More than 99% of the primers and most primer-dimers (<40 bp are removed).

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC
This page may contain text that has been machine translated.

Piktogramy

CorrosionExclamation mark
GHS05,GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H290,H302,H315,H319,H336

Klasyfikacja zagrożeń

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Central nervous system

Kod klasy składowania

8A - Combustible corrosive hazardous materials

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Protokoły

GenElute™ PCR Clean-Up Procedure

Follow this procedure to rapidly purify single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from excess primers, nucleotides, DNA polymerase, oil and salts using the GenElute™ PCR Clean-up Kit.

Procedura oczyszczania GenElute™ PCR

Zestaw GenElute™ PCR Clean-up Kit szybko oczyszcza produkty PCR z nadmiaru składników, zapewniając czyste próbki do dalszych zastosowań.

SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol

The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA

Protocol for DNA extraction & WGA Amplification from Soil Samples

Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the GenomePlex® Whole Genome Amplification Protocol is followed

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