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NA2110

Sigma-Aldrich

GenElute™ Bacterial Genomic DNA Kits

sufficient for 70 purifications

Synonim(y):

Bacterial Genomic DNA, bacterial DNA prep, bacterial DNA purification kit, bacterial gDNA extraction kit, bacterial gDNA isolation kit, bacterial gDNA purification kit, bacterial genomic DNA extraction kit, bacterial genomic DNA isolation kit, bacterial genomic DNA purification kit, Gen Elute

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About This Item

Kod UNSPSC:
41105501
NACRES:
NA.55

zastosowanie

sufficient for 70 purifications

Poziom jakości

200

metody

DNA purification: suitable

temp. przechowywania

15-25°C

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Opis ogólny

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Zastosowanie

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Cechy i korzyści

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required

Zasada

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Inne uwagi

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC
This page may contain text that has been machine translated.

Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki
  • W0263Wash Solution 1Karta charakterystyki
  • C2112Column Preparation SolutionKarta charakterystyki
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologyKarta charakterystyki
  • R6148RNase A solutionKarta charakterystyki

polecane

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Opis
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Numer produktu
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Cennik

P2308

Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

E1510

Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, for molecular biology, low EEO

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

R6148

RNase A solution

NA2100

GenElute™ Bacterial Genomic DNA Kits, sufficient for 10 purifications

NA2120

GenElute™ Bacterial Genomic DNA Kits, sufficient for 350 purifications

C2112

Column Preparation Solution

W0263

Wash Solution 1

SRE0005

Proteinase K from Tritirachium album, Reagents designed and manufactured under current ISO 13485 certification.

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H226,H290,H302,H315,H318,H334,H335,H336,H410

Klasyfikacja zagrożeń

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Central nervous system, Respiratory system

Kod klasy składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

77.0 °F - closed cup

Temperatura zapłonu (°C)

25 °C - closed cup

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

David B Adimpong et al.
Genome announcements, 1(2), e0009713-e0009713 (2013-03-30)
The Bacillus sonorensis L12 draft genome sequence is approximately 4,647,754 bp in size with a G+C content of 45.2%. Over 86% of the genome contains protein-encoding genes, including several gene clusters for de novo biosynthesis of the nonribosomal lipopeptides iturin, bacitracin
Hyunjin Yoon et al.
Infection and immunity, 79(1), 360-368 (2010-11-03)
Salmonella enterica serovar Typhimurium is an intracellular pathogen and a main cause of food-borne illness. In this study, a quantitative PCR (qPCR)-based competitive index (CI) method was developed to simultaneously compare the growth of multiple Salmonella strains. This method was
Hanan R Shehata et al.
Scientific reports, 7(1), 13479-13479 (2017-10-19)
In the animal microbiome, localization of microbes to specific cell types is well established, but there are few such examples within the plant microbiome which includes endophytes. Endophytes are non-pathogenic microbes that inhabit plants. Root hairs are single cells, equivalent
Joyce M Sakamoto et al.
PloS one, 9(7), e101389-e101389 (2014-07-16)
The most significant vector of tick-borne pathogens in the United States is Ixodes scapularis Say (the blacklegged tick). Previous studies have identified significant genetic, behavioral and morphological differences between northern vs. southern populations of this tick. Because tick-borne pathogens are
Manuel Simões et al.
Journal of basic microbiology, 47(3), 230-242 (2007-05-24)
Biocides generally have multiple biochemical targets. Such a feature easily entangles the analysis of the mechanisms of antimicrobial action. In this study, the action of the dialdehyde biocide ortho-phtalaldehyde (OPA), on bacteria, was investigated using the Gram-negative Pseudomonas fluorescens. The
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The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protokoły

Protokół zestawu GenElute™ Bacterial Genomic DNA Kit (NA2100, NA2110, NA2120)

Protokół GenElute™ Bacterial Genomic DNA Kit opisuje prosty i wygodny sposób izolacji czystego genomowego DNA z bakterii.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

Powiązane treści

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Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

Oczyszczanie DNA i RNA

Przegląd powszechnych technik i dalszych zastosowań do ekstrakcji i oczyszczania genomowego DNA, plazmidowego DNA i całkowitego RNA z komórek, tkanek, krwi, wirusów i innych rodzajów próbek.

Oczyszczanie DNA i RNA

Przegląd powszechnych technik i dalszych zastosowań do ekstrakcji i oczyszczania genomowego DNA, plazmidowego DNA i całkowitego RNA z komórek, tkanek, krwi, wirusów i innych rodzajów próbek.

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