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Merck

M9901

Sigma-Aldrich

Mutanolysin from Streptomyces globisporus ATCC 21553

lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified

Synonim(y):

Mutanolysin

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About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.54

pochodzenie biologiczne

bacterial (Streptomyces globisporus)

Poziom jakości

Postać

lyophilized powder

aktywność właściwa

≥4000 units/mg protein (biuret)

masa cząsteczkowa

23 kDa

przydatność

suitable for cell lysis

Zastosowanie

diagnostic assay manufacturing

Warunki transportu

wet ice

temp. przechowywania

−20°C

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Opis ogólny

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.

Zastosowanie

Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Działania biochem./fizjol.

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Definicja jednostki

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Postać fizyczna

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

Informacje prawne

Fiberfrax is a registered trademark of Unifrax Corp.
This page may contain text that has been machine translated.

Piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Resp. Sens. 1

Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Amelia T Soderholm et al.
Frontiers in cellular and infection microbiology, 8, 160-160 (2018-06-06)
Streptococcus pyogenes (Group A Streptococcus; GAS) commonly causes pharyngitis in children and adults, with severe invasive disease and immune sequelae being an infrequent consequence. The ability of GAS to invade the host and establish infection likely involves subversion of host
Mutanolysin for Improved Lysis and Rapid Protoplast Formation in Dairy Streptococci
Kondo, J. and L. McKay
Journal of Dairy Science, 65, 1428-1431 (1982)
Beth M Hayes et al.
Cell, 183(6), 1562-1571 (2020-12-12)
Ticks transmit a diverse array of microbes to vertebrate hosts, including human pathogens, which has led to a human-centric focus in this vector system. Far less is known about pathogens of ticks themselves. Here, we discover that a toxin in
Pierre Godessart et al.
Nature microbiology, 6(1), 27-33 (2020-11-04)
Gram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial
Stacey M Broomall et al.
Applied and environmental microbiology, 82(2), 596-607 (2015-11-15)
Effective microbial forensic analysis of materials used in a potential biological attack requires robust methods of morphological and genetic characterization of the attack materials in order to enable the attribution of the materials to potential sources and to exclude other

Protokoły

This procedure may be used for Mutanolysin products.

GenElute Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

Protokół GenElute™ Bacterial Genomic DNA Kit opisuje prosty i wygodny sposób izolacji czystego genomowego DNA z bakterii.

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