G1N350

GenElute^™ Mammalian Genomic DNA Miniprep Kits

sufficient for 350 purifications

• Synonim(y): Ekstrakcja gDNA ssaków, Izolacja gDNA ssaków, Oczyszczanie gDNA ssaków, Przygotowanie gDNA ssaków, Przygotowanie gDNA ssaków na krzemionce, miniprep gDNA, przygotowanie gDNA zwierząt
• Kod UNSPSC: 41105501
• NACRES: NA.55

Właściwości

• zastosowanie: sufficient for 350 purifications
• Poziom jakości: 200
• metody: DNA purification: suitable
• temp. przechowywania: 15-25°C

Opis

Opis ogólny

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.

Zastosowanie

GenElute^™ Mammalian Genomic DNA Miniprep Kits has been used:

• to extract DNA from fin samples
• to extract the BSTUI restriction enzyme from the DNA
• to isolate DNA from the blood samples extracted from caudal fin
• to extract tissue and hair follicle DNA

The purified genomic DNA is ready for immediate use in downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• ligation

Cechy i korzyści

• Expected yield: 25 μg from 2 x 10⁶ cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• Mechanical homogenization required: No

Zasada

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Inne uwagi

For additional information, please see www.sigma-aldrich.com/genomicdna.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Elementy zestawu są też dostępne oddzielnie

• C2112 Column Preparation Solution
• P2308 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
• R6148 RNase A solution

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS05,GHS07,GHS09
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H290,H302,H315,H318,H334,H335,H410
• Zwroty wskazujące środki ostrożności: P234 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 8A - Combustible corrosive hazardous materials
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable