H8280
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow
matrix fast-flow highly cross-linked 4% beaded agarose
Synonim(y):
N-Hydroxysuccinimidyl-Agarose
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About This Item
Polecane produkty
Formularz
isopropanol suspension
Poziom jakości
zakres etykietowania
16-23 μmol per mL
metody
protein array: suitable
Matryca
fast-flow highly cross-linked 4% beaded agarose
grupa aktywna macierzy
N-hydroxysuccinimide active ester
spacer macierzy
6 atoms (when ligands are coupled by displacement of NHS)
temp. przechowywania
2-8°C
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Opis ogólny
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.
Zastosowanie
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.
Cechy i korzyści
- Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
- High level of pre-activation, which gives a high degree of substitution of the selected ligand
- NHS coupling method enables chemically stable ligand attachment
Informacje prawne
Sepharose is a trademark of Cytiva
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
3 - Flammable liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
53.6 °F
Temperatura zapłonu (°C)
12 °C
Środki ochrony indywidualnej
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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