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Key Documents

E6754

Sigma-Aldrich

Epoxy-activated−Sepharose 6B

lyophilized powder

Synonim(y):

Epoxy-activated-Agarose

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About This Item

Numer MDL:
Kod UNSPSC:
41106500
NACRES:
NA.56

Postać

lyophilized powder

Poziom jakości

zakres etykietowania

19-40 μmol per mL

metody

affinity chromatography: suitable

macierz

Sepharose 6B

grupa aktywna macierzy

epoxy

MAR

through epoxy to hydroxyl

spacer macierzy

12 atoms

pęcznienie

1 g swells to 3.0-5.0 mL

wielkość cząstki

45—165 μm

temp. przechowywania

2-8°C

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Opis ogólny

when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Zastosowanie

Epoxy-activated-Sepharose is used in affinity chromatography, protein chromatography, and activated/functionalized matrices. Epoxy-activated-Sepharose has been used to obtain a monoclonal antibody against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B as well as to study B meningococcal disease.

Inne uwagi

Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Postać fizyczna

Lyophilized powder

Informacje prawne

Sepharose is a trademark of Cytiva
This page may contain text that has been machine translated.

zastąpiony przez

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

C A O'Brian et al.
Cancer research, 48(13), 3626-3629 (1988-07-01)
We have previously demonstrated that tamoxifen and related triphenylethylene compounds are potent inhibitors of protein kinase C (PKC). The present study demonstrates that PKC binds specifically and reversibly to the antiestrogen N-didesmethyltamoxifen when the drug is coupled to CNBr-activated agarose
Dong Li et al.
Veterinary research communications, 34(5), 445-457 (2010-06-01)
A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then
Adam Frankel et al.
RNA (New York, N.Y.), 9(7), 780-786 (2003-06-18)
The universal genetic code links the 20 naturally occurring amino acids to the 61 sense codons. Previously, the UAG amber stop codon (a nonsense codon) has been used as a blank in the code to insert natural and unnatural amino
Kasim Abass Askar et al.
Applied biochemistry and biotechnology, 165(1), 336-346 (2011-04-19)
The purpose of this study was to develop a protocol for the purification of acetylcholinesterase (AChE, acetylcholine acetylhydrolase, E.C.3.1.1.7) enzyme and to extend a purification method for further enzyme characterization. A further aim was to study whether the edrophonium's pharmacologic
A N Jina et al.
Biochemistry, 29(21), 5203-5209 (1990-05-29)
To synthesize an affinity matrix for isolation of D-myo-inositol 1,4,5-trisphosphate binding proteins, racemic 3-cyclohexene-1-carboxaldehyde was oxidized and converted to a mixture of trans-3,4-di-hydroxycyclohexane-1-carboxylic acid methyl ester isomers, which was phosphorylated and separated into (+-)-(1R,3R,4R)- and (+-)-(1R,3S,4S)-trans-3,4-bis[(diphenoxyphosphoryl)oxy]cyclohex an e-1- carboxylic acid

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