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H5666
Granulocyte-Macrophage Colony-Stimulating Factor human
HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonim(y):
GM-CSF human
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About This Item
Polecane produkty
Nazwa produktu
Granulocyte-Macrophage Colony-Stimulating Factor human, GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in HEK 293 cells
Próba
≥95% (SDS-PAGE)
Formularz
lyophilized powder
siła działania
≤5 ng/mL EC50
jakość
endotoxin tested
masa cząsteczkowa
dimer 15-36 kDa (glycosylated)
opakowanie
pkg of 5X10 μg
pkg of 10 μg
warunki przechowywania
avoid repeated freeze/thaw cycles
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤1 EU/mg
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... CSF2(1437)
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Opis ogólny
Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.
Zastosowanie
Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).
Działania biochem./fizjol.
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages. GM-CSF is implicated in inflammation associated disorder of the lung and renal system.
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Przestroga
Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
Postać fizyczna
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Uwaga dotycząca przygotowania
HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
Komentarz do analizy
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Informacje prawne
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Produkty
Read article on hematopoietic cytokines and hematopoiesis
Wymieniono cytokiny, których głównym działaniem wydaje się być stymulacja lub regulacja komórek krwiotwórczych, w tym GM-CSF, G-CSF, M-CSF, interleukiny, EPO i TPO.
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