H0135

Hydrocortisone

γ-irradiated, powder, BioXtra, suitable for cell culture

• Synonim(y): 11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M
• Wzór empiryczny (zapis Hilla): C₂₁H₃₀O₅
• Numer CAS: 50-23-7
• Masa cząsteczkowa: 362.46
• Beilstein: 1354819
• Numer WE: 200-020-1
• Numer MDL: MFCD00011654
• Kod UNSPSC: 12352209
• Identyfikator substancji w PubChem: 24895394
• NACRES: NA.77

Właściwości

• pochodzenie biologiczne: synthetic (organic)
• Poziom jakości: 200
• sterylność: γ-irradiated
• linia produktu: BioXtra
• Próba: ≥80% (HPLC)
• Postać: powder
• metody: cell culture | mammalian: suitable
• mp: 211-214 °C (lit.)
• rozpuszczalność: ethanol: 1 mg/mL, clear
• Warunki transportu: ambient
• temp. przechowywania: room temp
• ciąg SMILES: [H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
• InChI: 1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
• Klucz InChI: JYGXADMDTFJGBT-VWUMJDOOSA-N
• informacje o genach: human ... ABCB1(5243) , CYP3A4(1576) , NR3C1(2908) , SERPINA6(866) ; mouse ... Abcb1a(18671) , Abcb1b(18669) , Ifng(15978) , Nos2(18126) ; rat ... Ar(24208) , Nr3c1(24413)

Opis

Opis ogólny

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Zastosowanie

Hydrocortisone is used for the following applications:

• Used in myogenic induction and identification
• Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
• Used in adipogenic differentiation (one of the components in adipogenic medium)
• Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
• The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
• Used in differentiation assays of melanoma spheroid cells

Działania biochem./fizjol.

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na²⁺ retention potency.

Postać fizyczna

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Rekonstytucja

To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H360Df,H373
• Zwroty wskazujące środki ostrożności: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Klasyfikacja zagrożeń: Repr. 1A - STOT RE 2
• Organy docelowe: Adrenal gland,Bone marrow
• Kod klasy składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges