D5546

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonim(y): DME, DMEM
• Numer MDL: MFCD00217342
• Kod UNSPSC: 12352207
• NACRES: NA.75

Właściwości

• Poziom jakości: 400
• sterylność: sterile-filtered
• Postać: liquid
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: endotoxin, tested
• komponenty: NaHCO₃: yes; phenol red: yes; glucose: low; HEPES: no; sodium pyruvate: yes; L-glutamine: no
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium. 

Zastosowanie

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

• human osteosarcoma cells and HeLa cells
• primary human mesenchymal stromal cells (hBMSCs)
• human embryonic kidney cell lines

Rekonstytucja

Supplement with 0.584 g/L glutamine.

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable