D5671

DMEM - high glucose

With sodium Bicarbonate, without ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

• Synonim(y): DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
• Kod UNSPSC: 12352207
• NACRES: NA.75

Właściwości

• product name: Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
• Poziom jakości: 400
• sterylność: sterile-filtered
• Postać: liquid
• metody: cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• zanieczyszczenia: endotoxin, tested
• komponenty: L-glutamine: no; glucose: high; NaHCO₃: yes; sodium pyruvate: no; phenol red: yes; HEPES: no
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Zastosowanie

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Rekonstytucja

Supplement with 0.584 g/L L-glutamine.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable