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Merck

D5648

Sigma-Aldrich

DMEM - high glucose

With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture

Synonim(y):

DME, Zmodyfikowana pożywka Eagle'a Dulbecco - wysoki poziom glukozy

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.75

Nazwa produktu

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Formularz

powder

metody

cell culture | mammalian: suitable

komponenty

phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Zastosowanie

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue

Ilość

Formulated to contain 13.4 grams of powder per liter of medium.

Rekonstytucja

Supplement with 3.7 g/L sodium bicarbonate.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Certyfikaty analizy (CoA)

Lot/Batch Number

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Ana M S Cardoso et al.
Human molecular genetics, 28(16), 2738-2751 (2019-05-16)
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Jelka Gabrilovac et al.
Immunobiology, 222(2), 327-342 (2016-09-30)
Dipeptidyl peptidase 9 (DPP9) is a relatively new member of the DPPIV family of prolyl dipeptidases which is ubiquitously expressed. Its role in regulation of immune responses and proliferation of epithelial carcinoma cells was reported. There is no data on
Lingling Lu et al.
Acta histochemica, 121(6), 695-703 (2019-06-27)
Renal fibrosis is a common mechanism that leads to all kidney diseases and Epithelial-mesenchymal transition (EMT) is considered as one of the potential mechanisms of renal fibrosis. Inhibitor of growth 4 (ING4) was reported to involve in several diseases; especially
Ayako Sasaki et al.
Human cell, 29(1), 37-45 (2015-11-11)
Of two stereoisomers of glucose, only D- and not L-glucose is abundantly found in nature, being utilized as an essential fuel by most organisms. The uptake of D-glucose into mammalian cells occurs through glucose transporters such as GLUTs, and this
Denise Riquelme et al.
Frontiers in cellular neuroscience, 12, 12-12 (2018-02-15)
TRPM4 is a Ca

Produkty

Cell culture protocol for passaging and splitting suspension cell lines

Protokół hodowli komórkowej do pasażowania i dzielenia zawiesinowych linii komórkowych

Protokoły

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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