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D7777
Dulbecco′s Modified Eagle′s Medium - high glucose
With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture
Synonim(y):
DME, DMEM
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About This Item
Kod UNSPSC:
12352207
NACRES:
NA.75
Polecane produkty
Poziom jakości
Formularz
powder
metody
cell culture | mammalian: suitable
komponenty
glucose: high
sodium pyruvate: yes
NaHCO3: no
HEPES: no
phenol red: yes
L-glutamine: yes
Warunki transportu
ambient
temp. przechowywania
2-8°C
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Zastosowanie
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Ilość
Formulated to contain 13.5 grams of powder per liter of medium.
Rekonstytucja
Supplement with 3.7 g/L sodium bicarbonate
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Kod klasy składowania
13 - Non Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
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Protokoły
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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