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Merck

B1522

SAFC

Basal Medium Eagle

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonim(y):

BME

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.75

Nazwa produktu

Basal Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterylność

sterile-filtered

Formularz

liquid

metody

cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

komponenty

L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

Eagle′s Basal Medium (BME) is one of the most used synthetic cell culture media. The tissue culture association recommends that the term "Eagle′s Basal Medium" be used only to refer to the formula developed to support HeLa cells. BME has previously been used in monolayer culture studies to assess the growth response of normal (WI-38) and transformed (mouse and HeLa) cells. BME is the precursor of Dulbecco′s Modified Eagle′s medium (DME) and Eagle′s Minimum Essential Medium (MEM).

Zastosowanie

Basal Medium Eagle has been used:
  • as a component in the culture medium for cochlear organotypic cultures and salicylate treatment
  • to maintain cerebellar granule neurons (CGNs) to determine if there is a system that regulates the distribution and/or group dynamics of axonal mitochondria
  • in the preparation of cortical slices, serum-free medium, and slice culture medium for slice overlay assay to study the influence of extracellular signals on neuronal development

Rekonstytucja

Supplement with 0.292 g/L L-glutamine.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Odwiedź Bibliotekę dokumentów

Franck Polleux et al.
Science's STKE : signal transduction knowledge environment, 2002(136), pl9-pl9 (2002-06-13)
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Franck Polleux et al.
Science's STKE : signal transduction knowledge environment, 2002(136), pl9-pl9 (2002-06-13)
We have developed a technique that allows characterization and identification of extracellular signals that regulate various aspects of neuronal differentiation. In this in vitro assay, dissociated cells isolated from the developing cerebral wall are labeled and cultured over organotypic cortical
Lizhen Wang et al.
Neurobiology of aging, 29(3), 357-367 (2006-12-13)
beta-Site APP cleavage enzyme 1 (BACE1) is the beta-secretase responsible for generating amyloid-beta (A beta) peptides in Alzheimer's disease (AD). Previous studies suggest that activation of protein kinase C (PKC) modulates the beta-secretase-mediated cleavage of APP and reduces the production

Produkty

Cell culture protocol for passaging and splitting suspension cell lines

Protokół hodowli komórkowej do pasażowania i dzielenia zawiesinowych linii komórkowych

Wapń jest jonowo stabilnym dwuwartościowym kationem o ważnych korzystnych i toksycznych właściwościach w hodowli komórkowej. Jest składnikiem szerokiej gamy pożywek do hodowli komórkowych.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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